LOCUS       sequence-405510-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Maynard AG, Pohl NK, Mueller AP, Petrova B, Wong AYL, Wang P, 
            Culhane AJ, Brook JR, Hirsch LM, Hoang N, Kirkland O, Braun T, 
            Ducamp S, Fleming MD, Li H, Kanarek N
  TITLE     Folate depletion induces erythroid differentiation through 
            perturbation of de novo purine synthesis.
  JOURNAL   Sci Adv. 2024 Feb 2;10(5):eadj9479. doi: 10.1126/sciadv.adj9479. 
            Epub 2024 Jan 31.
  PUBMED    38295180
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-2"; volume: "10(5):eadj9479. doi"; pages: " 
            10.1126/sciadv.adj9479. Epub 2024 Jan 31"
COMMENT     Sequence Label: pLenti-sgAMPKa2-Cas9-GFP
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        67..142
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    190..307
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        376..587
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             616..639
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             646..666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaag atcggacact
       61 acgtgcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
      121 aaaagtggca ccgagtcggt gcttttttga attctagatc ttgagacaaa tggcagtatt
      181 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
      241 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
      301 aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc tcgagtggct
      361 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      421 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      481 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      541 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggtg tcgtgacgcg
      601 ggatccgcca ccatggatta caaagacgat gacgataaga tggccccaaa gaagaagcgg
      661 aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg cctggacatc
      721 ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa
      781 ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg
      841 ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga
      901 tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc
      961 aa
//
