LOCUS       sequence-405522-        4252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 27877 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-27877
FEATURES             Location/Qualifiers
     source          1..4252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3407..3995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3487..3506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4086..4252,1..625))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
       61 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
      121 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
      181 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
      241 tgtcgatcgt ggctggctcg aagataccag caagaatgtc attgcgctgc cattctccaa
      301 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
      361 tgacttctac agcgcggaga atctcactct ctccagggga agccgaagtt tccaaaaggt
      421 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
      481 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
      541 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
      601 cttcggcgat caccgcttcc ctcatgcgaa acgatcctca tcctgtctct tgatcagata
      661 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
      721 gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctaagcttg
      781 catgcctgca agtcgactct agaggagcgg tgatcacagg cagcaacgct ctgtcatcgt
      841 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
      901 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
      961 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     1021 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctcagg aggtcgatat
     1081 cagccctcac ctccaaatcg tcaagccaag atgagtgagt gtcagtgagg atttgtactt
     1141 tccaaaggct tgatcactgg ctcatccaaa acgatttttt gtagtggaat cctacgtctc
     1201 gtactgttga taagaaaagc tatttatggt ctatgattga acgacgtaag aacggtcctg
     1261 tgcgaattct gtaaaactac ggagacacaa attcaagtgg tactctctaa ttaagctggt
     1321 ataggccttg aaatgactga cgcgccactg ttctccgatg agccaatcaa aacctttcaa
     1381 tcttacctgg ccgttgcctt ctctcgaaca attaatctgg agttctgaat tcgatttaat
     1441 attttctgtc tatatcgacg caatcgtatt ggatgattaa gcttcgatag gattctagtg
     1501 gctgataatg tcgtttgatt gtgcaagcgt gtcaattgcg ataatagagt tgatgattgg
     1561 cccagggcaa ttatatagga tcaaccgtcc tgtgaagttg gtaatgattc tacataccgg
     1621 aaactgctac gctgtgcttc tactaaaccg tccctcacac ctctttgatc agatcggaat
     1681 ttccgtttgc acccacgcgg atccgacctc cgtaactttc ataaaactct gtcgacccgt
     1741 gaagtcgttc tctcctctgt gacacactca tcctctcagc attcaccaat gcgagaccac
     1801 caggatacat agattaccac aactccgagc ccttccaccc acagaatcag gggataacgc
     1861 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1921 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
     1981 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
     2041 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
     2101 atgcgaaacg atcctcatcc tgtctcttga tcagatattg atcccctgcg ccatcagatc
     2161 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
     2221 gccccagctg gcaattccgg ttcgcttgct aagcttgcat gcctgcaagt cgactctaga
     2281 ggagcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
     2341 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
     2401 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
     2461 gggctgcctg tatcgagtgg tgattttgtg ccgagctcat tactcgcatc cattctcagg
     2521 ctgtctcgtc tcgtctcagg tctcaggagg tcgatatcag ccctcacctc caaatcgtca
     2581 agccaagatg agtgagtgtc agtgaggatt tgtactttcc aaaggcttga tcactggctc
     2641 atccaaaacg attttttgta gtggaatcct acgtctcgta ctgttgataa gaaaagctat
     2701 ttatggtcta tgattgaacg acgtaagaac ggtcctgtgc gaattctgta aaactacgga
     2761 gacacaaatt caagtggtac tctctaatta agctggtata ggccttgaaa tgactgacgc
     2821 gccactgttc tccgatgagc caatcaaaac ctttcaatct tacctggccg ttgccttctc
     2881 tcgaacaatt aatctggagt tctgaattcg atttaatatt ttctgtctat atcgacgcaa
     2941 tcgtattgga tgattaagct tcgataggat tctagtggct gataatgtcg tttgattgtg
     3001 caagcgtgtc aattgcgata atagagttga tgattggccc agggcaatta tataggatca
     3061 accgtcctgt gaagttggta atgattctac ataccggaaa ctgctacgct gtgcttctac
     3121 taaaccgtcc ctcacacctc tttgatcaga tcggaatttc cgtttgcacc cacgcggatc
     3181 cgacctccgt aactttcata aaactctgtc gacccgtgaa gtcgttctct cctctgtgac
     3241 acactcatcc tctcagcatt cacaaatgcg agaccaccag gatacataga ttaccacaac
     3301 tccgagccct tccacccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3601 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3781 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3841 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3901 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     4081 gattattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
     4141 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     4201 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc gg
//
