LOCUS       sequence-405524-        2832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 41423 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-fbaC5
FEATURES             Location/Qualifiers
     source          1..2832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1987..2575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2067..2086)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2666..2832,1..625))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
       61 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
      121 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
      181 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
      241 tgtcgatcgt ggctggctcg aagataccag caagaatgtc attgcgctgc cattctccaa
      301 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
      361 tgacttctac agcgcggaga atctcactct ctccagggga agccgaagtt tccaaaaggt
      421 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
      481 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
      541 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
      601 cttcggcgat caccgcttcc ctcatgcgaa acgatcctca tcctgtctct tgatcagata
      661 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
      721 gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctaagcttg
      781 catgcctgca agtcgactct agaggagcgg tgatcacagg cagcaacgct ctgtcatcgt
      841 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
      901 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
      961 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     1021 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctccgg agtcacagtc
     1081 gacgttccta cctcggagtc tagagcgcta ctttgttgcc ggggactttt tattggtaga
     1141 tgaacagata cgaacagatt ctccgagtga tactgttagt cgcgagtgct ttttaagctc
     1201 gctctcttgc gatagattta gattaacctt atctctgctg caaagaaacg agataccttc
     1261 aaacatgatt caaactcaaa aaatattgtc gatttttgga tttttgaaat tggtggcgag
     1321 actcggtcct gtgccgagag tcgggacgac catgacggtc acgggagggc acacagccgg
     1381 agtgtgtctg tacttctctg atgcacttac aggaacggcg caggattcat tctacccggc
     1441 gatagccagc gatacggaaa gtaaaaaaat gcttccgatg gcgggtgtgg acacgtgcca
     1501 accgaccagg actcaagttc tcgatcatgc tcgaaagaag tccgcgtgat tcttacgggc
     1561 gaacgtaggc gaattggctg tcgtaaattc cttctttggc gttctgcatc tttacgtaac
     1621 caacgtcaac gtcacctgta cgaaacgtac aaactgactg gcttcttttc tgtccaagat
     1681 cttcgtgcac aacgcaacgc gttaaactct acaccatgag attctccttg caatcttcac
     1741 ttgcagttct tctggttttg caagccagtc acgcagctgc tttttcggcc cccgtctcat
     1801 cgagcaatgg aaagaacgga attagatcgt tcgctccgtt gagcaatgcg agaccaccag
     1861 gatacataga ttaccacaac tccgagccct tccacccaca gaatcagggg ataacgcagg
     1921 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1981 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2041 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2101 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2161 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2221 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2281 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2341 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2401 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2461 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2521 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2581 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2641 tttggtcatg cattctaggt gattattatt tgccgactac cttggtgatc tcgcctttca
     2701 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     2761 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     2821 ttgcccagtc gg
//
