LOCUS       sequence-405525-        2732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 39236 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-CaMK2
FEATURES             Location/Qualifiers
     source          1..2732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             871..1662
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1753..2341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2242..2261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagtgcaaga ggcatgggac tacgtggtac cagcttttac ggttagatct gcgaaaacga
       61 cgccttgtcg aacgtacgta cctgcactgg tcaagagcct ctattggcct ttccacaaaa
      121 catacatgcg gtagccagaa cactcccttt tcgaaatcta aacatcgtgt tagttcacat
      181 tcatgtgaat gaaaaagtta gctaatagat gtccgaagta acaaaacacg taatcgcaga
      241 cggcgattga tgacgtagct gtgtacgtca cgttccaaac ggacagtgca agtgacggtc
      301 cgtcacacaa aatgattcga gcctacccta cccaattcgg tcaagtttca attttggcgc
      361 gatgtaggac attgttataa gagatgaacg agattgcgac tgggctttcg aacgcgttct
      421 cgtctcctga gacctgagac gagacgagac agcctgagaa tggatgcgag taatgagctc
      481 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
      541 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
      601 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
      661 gacagagcgt tgctgcctgt gatcaccgct cctctagagt cgacttgcag gcatgcaagc
      721 ttagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
      781 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaatatct
      841 gatcaagaga caggatgagg atcgtttcgc atgagggaag cggtgatcgc cgaagtatcg
      901 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
      961 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     1021 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     1081 ttggaaactt cggcttcccc tggagagagt gagattctcc gcgctgtaga agtcaccatt
     1141 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     1201 gaatggcagc gcaatgacat tcttgctggt atcttcgagc cagccacgat cgacattgat
     1261 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     1321 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     1381 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     1441 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     1501 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     1561 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     1621 cactacgtga aaggcgagat caccaaggta gtcggcaaat aataatcacc tagaatgcat
     1681 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1741 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1801 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1861 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1921 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1981 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2041 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2101 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2161 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2221 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2281 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2341 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2401 gttctttcct gcgttatccc ctgattctgt gggtggaagg gctcggagtt gtggtaatct
     2461 atgtatcctg gtggtctcgc attcttgttt cgagtacggt tttgttctca attgctacta
     2521 ggtcttcaaa caaagtgaaa gtttgattag aattgtaagc cttcccggcc gtgttcaatt
     2581 tatagttagt gatgtcatcc caatttggaa agaaaggctt ttcacgcgtg tgtatttacg
     2641 tagggacgag cctaatttcg tagatcaggc caatttcttc tattctattc aaagtatgcc
     2701 tacaaaattt cgttccaagg aacgaagaga gt
//
