LOCUS       sequence-405526-        2992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 29812 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-act1
FEATURES             Location/Qualifiers
     source          1..2992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      826..1414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1315..1334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2936..2992,1..735)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
       61 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
      121 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
      181 agtgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
      241 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgct
      301 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
      361 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
      421 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
      481 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
      541 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
      601 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
      661 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
      721 gtagtcggca aataataatc acctagaatg catgaccaaa atcccttaac gtgagttttc
      781 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
      841 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
      901 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
      961 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1021 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1081 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1141 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1201 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1261 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1321 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1381 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1441 gttccttgcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1501 tgtgggtgga agggctcgga gttgtggtaa tctatgtatc ctggtggtct cgcattgatg
     1561 ggatagagag gtgttgaaga atacggcggt gtaaggcgaa ggacaggaag aactttcgct
     1621 tggcaaggca aaagttacgt cacggtagat ttgtgaggct gctggtgctg tccaaaacgt
     1681 tgcgttggat cctgcttgga atgaccgttt tcgcaaattt tgaaagcttc ggcgatgacc
     1741 gctaaaatga ccctcttttg aagaaacgat ccccttgtgg tccccgtcgt acttcatgta
     1801 cagaatgcta cgtaagcaaa acttggatta ggcggaaaaa gtgaggctgt ccgatcgtgg
     1861 ttgaaattga ggatggcgtt tccatccttg gaacaccata ctgacgactg gagaactatt
     1921 tacgtcccga aaactatttg ctccaattga aggccagtat gaacaatgga tacctaaacc
     1981 ttacagagtg aatcccgtgc gaactgtgtg gtagaatctc gttagcgcca acacgtttcc
     2041 tgcgaaaaat agtacgctag tagaattata gagatagctc atattcagac aaggaaacaa
     2101 gactgaaaag atagccacga caaaaaagtt catgttgcaa gaggtcggga tggatgccag
     2161 agtcgcccta gtctgtatgc tgaaattaat tgtttcacat gatatcttta agttcattct
     2221 cgcaattgct ggtcttgctg gaaaaggcag cagaaagaaa ggcgcatcat ggacaatgaa
     2281 tctaacccat ttatattggc tagcttattt tcattgtaca ttcattgtta actcttttgc
     2341 tttcaacatt ccactctcat tgttgacaga ttattgtcaa tatcctgtct attccacaat
     2401 tgttcacaaa gccatatgga aaaatataag ctgctagctt gacacagctc cggagcccga
     2461 tgtcagcatc aaatatactt tcgtcgacct ccggagacct gagacgagac gagacagcct
     2521 gagaatggat gcgagtaatg agctcggcac aaaatcacca ctcgatacag gcagcccatc
     2581 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     2641 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     2701 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca ccgctcctct
     2761 agagtcgact tgcaggcatg caagcttagc aagcgaaccg gaattgccag ctggggcgcc
     2821 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     2881 ctgatggcgc aggggatcaa tatctgatca agagacagga tgaggatcgt ttcgcatgag
     2941 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tc
//
