LOCUS       sequence-405527-        2857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 48707 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-sit4
FEATURES             Location/Qualifiers
     source          1..2857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             388..1179
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1270..1858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1759..1778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
       61 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
      121 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
      181 agagcgttgc tgcctgtgat caccgctcct ctagagtcga cttgcaggca tgcaagctta
      241 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
      301 gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc aatatctgat
      361 caagagacag gatgaggatc gtttcgcatg agggaagcgg tgatcgccga agtatcgact
      421 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
      481 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
      541 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
      601 gaaacttcgg cttcccctgg agagagtgag attctccgcg ctgtagaagt caccattgtt
      661 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
      721 tggcagcgca atgacattct tgctggtatc ttcgagccag ccacgatcga cattgatctg
      781 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
      841 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
      901 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
      961 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     1021 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     1081 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     1141 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat aatcacctag aatgcatgac
     1201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1321 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1381 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1441 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1501 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1561 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1621 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1681 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1741 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1801 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1861 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1921 ctttcctgcg ttatcccctg attctgtggg tggaagggct cggagttgtg gtaatctatg
     1981 tatcctggtg gtctcgcatt tgttgtatta ttgcgcttac tttttctgta agcgagagag
     2041 ttagcgtttg ggcttgactt ggttggatgc accttgatca aactgcgtcc agaacaaata
     2101 tgtaagtact gggtttccta gttggaaggt gagcattgaa cttgttcgcc agtatggcaa
     2161 gcattctgct tttggcggtt tcgctaaatt cagatgtcgg ttaacttcaa tcttggaaag
     2221 catagtaatc gaactattta cagttatatc taccgttacc aaggaatttt tcgcgagcta
     2281 ggaatttact tagagcttgc gcgctcgacg aattttgtgg ataagcatag taaaagaccg
     2341 actacggtta tttagtcagc ttgaaaaacc catgattgaa tagtcccaat ggatatgtcg
     2401 cgtacatgga acgaaactgg atttgtccct ttcgcacgaa tcatatagtt gaatgtttgc
     2461 aagctaataa atggttcgtc gtggacgaca gacattttta tgcttgcatt ccccgtgaca
     2521 ctccgagctt ttcgtattgt cgtcgcaggc gcaaaagcat cccaacacct cgtgcggtgt
     2581 tcctaccgct gcacctaaca gtcaggaaca tttgtccgct cggcgttgta cagcgtctta
     2641 ttcttccgac acgcgttttt gtccataaga agcatatttg tatgattagt aacgttacat
     2701 gcaaaaattt gacgatttca cgaactgcat tgatgatgca aagtctaacc aagctcggct
     2761 ggacacggtg aagctcgtca atgactgttg atgatacctc ctgagacctg agacgagacg
     2821 agacagcctg agaatggatg cgagtaatga gctcggc
//
