LOCUS       sequence-405529-        2520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 54101 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-NRT
FEATURES             Location/Qualifiers
     source          1..2520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1677..2265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1757..1776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2356..2520,1..627))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
       61 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
      121 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
      181 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
      241 aatgtcgatc gtggctggct cgaagatacc agcaagaatg tcattgcgct gccattctcc
      301 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
      361 ggtgacttct acagcgcgga gaatctcact ctctccaggg gaagccgaag tttccaaaag
      421 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
      481 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
      541 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
      601 tacttcggcg atcaccgctt ccctcatgcg aaacgatcct catcctgtct cttgatcaga
      661 tattgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
      721 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctaagct
      781 tgcatgcctg caagtcgact ctagaggagc ggtgatcaca ggcagcaacg ctctgtcatc
      841 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
      901 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
      961 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
     1021 ctcattactc gcatccattc tcaggctgtc tcgtctcgtc tcaggtctca ggagtagtat
     1081 tccagtgctt cccgcaggtg gtcatataaa acatgtttac atacttagat ttttcctact
     1141 tataaatgtc tgattatctg acgcccgact gactcacaaa atacggtagg tagtgcttct
     1201 atcataagta tagggagcaa accattttgt ctccgcctat agaatatctc acgaccgccg
     1261 gccttttagg ttccaccgtt tcgcaacttc cgtccagctc gacttcgttc ccgaagtcga
     1321 agcctatttg ttccccagaa aaagaggaag catcttcttc ctatggtaga cgacacgtcg
     1381 gtccagtaca ctttctcaat acggaacaaa atatccgcca ctcgaagttt cgatatctct
     1441 tttcagcatt cgatagcctt acactcacaa atcttgtgag catcacttcg acaatcaata
     1501 aagcggccag aacgaaagta ataaaactac aatcaatgcg agaccaccag gatacataga
     1561 ttaccacaac tccgagccct tccacccaca gaatcagggg ataacgcagg aaagaacatg
     1621 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1681 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1741 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1801 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1861 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1921 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1981 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2041 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2101 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2161 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2221 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2281 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2341 cattctaggt gattattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
     2401 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
     2461 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
//
