LOCUS       sequence-405531-        2601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 21592 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-lysA
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             700..1491
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1582..2170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2071..2090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggccaaaga ctaggttatg tttacttgga cacgtaggaa tgagcgcata tcccgtttcc
       61 gaacgtcgta gtagcataag cagtaactga ctgtgacatt tatgggattt gttgtttcgt
      121 catccaagtg actaacgcaa gttctcttag gtagccattt tctgtctttc tttggtcatg
      181 gcgacaatgg cgaaaatcta catagatcca ccctgactga aagcaattca aacttcggtc
      241 ccagttctgc aactcctgag acctgagacg agacgagaca gcctgagaat ggatgcgagt
      301 aatgagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
      361 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
      421 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
      481 tgtaacgatg acagagcgtt gctgcctgtg atcaccgctc ctctagagtc gacttgcagg
      541 catgcaagct tagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
      601 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
      661 tcaatatctg atcaagagac aggatgagga tcgtttcgca tgagggaagc ggtgatcgcc
      721 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
      781 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
      841 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
      901 aacgaccttt tggaaacttc ggcttcccct ggagagagtg agattctccg cgctgtagaa
      961 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     1021 caatttggag aatggcagcg caatgacatt cttgctggta tcttcgagcc agccacgatc
     1081 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     1141 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     1201 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     1261 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     1321 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     1381 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     1441 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata ataatcacct
     1501 agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg ggtggaaggg ctcggagttg
     2281 tggtaatcta tgtatcctgg tggtctcgca ttcgtggaag tgctttggtg cgtgtggaag
     2341 gaaggagatt gaggataaac gaaaacccaa gactgttagt gtgcgtttag gagtaagcaa
     2401 gaaattgcaa cgggtgtggc aggttcactg tcccaagcgt cttcggccgc tcactgtctg
     2461 tcaactttct tccacacgaa gtcacagtca gtgctttttg tgcgtttgta tccgatggcc
     2521 gatggcgtcc acttcggccg agtcgatgcc tgtctgggct ggtgggacgg aaggaacgcg
     2581 cggccccagt cgaggcgttt a
//
