LOCUS       sequence-405532-       11778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 11778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO
FEATURES             Location/Qualifiers
     source          1..11778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    123..696
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    146..698
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(290..307)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     517..536
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             698..718
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1325..1837
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1847..1900
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2921..2974
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2981..3715
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     3118..3139
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3242..3263)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3503..3522)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3960..4548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4013..4033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4431..4442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4755..5388
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5516..5532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5516..5532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5529..5551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5540..5556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5564..5570,5571..5588,5589..5594))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5609..5630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5747..5764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5918..6506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5998..6017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6677..7468,7469..7537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7300..7319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7538..7642)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7690..7824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7727..7746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7781..7800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7992..8625
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8672..8797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9294..9527
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9712..9756
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10019..10134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        10192..10495
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10496..10699
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10650..10670
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10696..10720
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggtctacact cactttacct gtgccactga caccaaaaac gtgcagtttg tttttgatgc
       61 tgttacagat gtcatcatta aaaacaactt aaaggaatgt gggctttact gagcggccgc
      121 cgcccctctc cctccccccc ccntaacgtt actggccgaa gccgcttgga ataaggccgg
      181 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
      241 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
      301 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
      361 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
      421 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
      481 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
      541 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
      601 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
      661 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gggagtagca agagcaagac
      721 gtcgaattcc ggaatcaagc ttcttgattg cgaccaagaa ctctacaaga acttccctct
      781 ggtgatctct gagcgctggc agcaggaagt ggcggaaaca gtttatgaag cagtaaatgc
      841 agacacggat aaaatcgagg ccaggaagag agctaaaaat aagcagcttg gccacgaaga
      901 agattacgcc ctgggaagag actgcatcgt gcacgggtac atgctgaagc tggggaaccc
      961 tttcctgacc cagtggcagc gccgctattt ttacctcttt ccgaacagac ttgagtggag
     1021 aggagaaggc gagtcgcgac aaagtttact gacaatggaa cagattgtgt ccgtggaaga
     1081 aactcagatt aaagacaaaa agtgcatttt gttgagaata aaaggaggga agcagttcgt
     1141 tttgcagtgt gagagtgacc cagagtttgt gcagtggaag aaagagctga cggagacatt
     1201 catggaggcc cagcggctgc tacggcgagc ccccaagttc ctcaacaaat cccgctcagc
     1261 cgtcgtggaa ctctcaaagc ctcccctctg ccataggaac agcaacggcc tcggaggtgg
     1321 atccatggtc ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa
     1381 cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc
     1441 cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca
     1501 tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt
     1561 taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact atggcacact
     1621 ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat
     1681 cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg gcaacaaaat
     1741 tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa ccatcaacgg
     1801 agtgaccggc tggcggctgt gcgaacgcat tctggcggga tcaggagaag gtagaggaag
     1861 tctactaaca tgcggggacg tggaggaaaa tcccggccca atgggggagc ttgaccagtt
     1921 acggcaggag gccgagcaac ttaagaacca gattcgagac gccaggaaag catgtgcaga
     1981 tgcaactctc tctcagatca caaacaacat cgacccagtg ggaagaatcc aaatgcgcac
     2041 gaggaggaca ctgcgggggc acctggccaa gatctacgcc atgcactggg gcacagactc
     2101 caggcttctc gtcagtgcct cgcaggatgg taaacttatc atctgggaca gctacaccac
     2161 caacaaggtc cacgccatcc ctctgcgctc ctcctgggtc atgacctgtg catatgcccc
     2221 ttctgggaac tatgtggcct gcggtggcct ggataacatt tgctccattt acaatctgaa
     2281 aactcgtgag gggaacgtgc gcgtgagtcg tgagctggca ggacacacag gttacctgtc
     2341 ctgctgccga ttcctggatg acaatcagat cgtcaccagc tctggagaca ccacgtgtgc
     2401 cctgtgggac atcgagaccg gccagcagac gaccacgttt accggacaca ctggagatgt
     2461 catgagcctt tctcttgctc ctgacaccag actgttcgtc tctggtgctt gtgatgcttc
     2521 agccaaactc tgggatgtgc gagaaggcat gtgccggcag accttcactg gccacgagtc
     2581 tgacatcaat gccatttgct tctttccaaa tggcaatgca tttgccactg gctcagacga
     2641 cgccacctgc aggctgtttg accttcgtgc tgaccaggag ctcatgactt actcccatga
     2701 caacatcatc tgcgggatca cctctgtctc cttctccaag agcgggcgcc tcctccttgc
     2761 tgggtacgac gacttcaact gcaacgtctg ggatgcactc aaagccgacc gggcaggtgt
     2821 cttggctggg catgacaacc gcgtcagctg cctgggcgtg actgacgatg gcatggctgt
     2881 ggcgacaggg tcctgggata gcttcctcaa gatctggaac gagggcagag gaagtcttct
     2941 aacatgcggt gacgtggagg agaatcccgg ccctaccggt atggacggcg gcgtgcagct
     3001 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3061 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3121 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3181 gggtggcagc ggtggcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3241 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3301 gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc
     3361 cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta
     3421 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3481 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3541 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3601 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc
     3661 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgagtccgg
     3721 actcagatct atggccagca acaacaccgc cagcatagca caagccagga agctggtaga
     3781 gcagcttaag atggaagcca atatcgacag gataaaggtg tccaaggcag ctgcagattt
     3841 gatggcctac tgtgaagcac atgccaagga agaccccctc ctgacccctg ttccggcttc
     3901 agaaaacccg tttagggaga agaagttttt ctgtgccatc ctttaaacgc gtctggaaca
     3961 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4021 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4081 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4141 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4201 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4261 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4321 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4381 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4441 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4501 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     4561 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     4621 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4681 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4741 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     4801 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     4861 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     4921 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     4981 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     5041 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     5101 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     5161 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     5221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5401 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     5461 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5521 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5581 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5641 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5701 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7681 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7741 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7801 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     7861 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     7921 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     7981 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     8041 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     8101 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8161 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     8221 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     8281 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     8341 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     8401 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     8461 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8521 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8581 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8641 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9121 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     9181 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9241 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9301 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9361 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9421 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9481 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9541 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9601 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9661 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9721 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9781 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9841 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9901 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     9961 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgcctt
    10021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    10081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    10141 ttattacagg gacagcagag atccagttta tcgataagct tgggagttcc gcgttacata
    10201 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    10261 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    10321 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
    10381 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    10441 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
    10501 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
    10561 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
    10621 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
    10681 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
    10741 ctgttttgac ctccatagaa gacaccgact ctactagagg atctatttcc ggtgaattcc
    10801 tcgagactag ttctagagcg gccgcatggg ctgcacgttg agcgccgagg acaaggcggc
    10861 ggtggagcgg agtaagatga tcgaccgcaa cttgcgggag gacggagaga aagcggccaa
    10921 agaagtgaag ctgctgctgc tcggcgctgg agaatctggt aaaagtacta ttgtgaaaca
    10981 gatgaaaatc attcatgagg atggctattc cgaggacgag tgtaagcagt ataaagttgt
    11041 cgtctacagc aataccattc agtccatcat tgcaatcata agagccatgg gacggttgaa
    11101 gattgatttt ggggaagctg ccagagcgga tgatgcccga cagttatttg ttttagctgg
    11161 cagtgctgag gaaggagtca tgacttcaga actagcaggc gtgattaaac gtttatggcg
    11221 agatggcggg gtgcaggcat gcttcagcag gtccagggaa tatcagctca atgattctgc
    11281 ttcatattac ctaaatgatt tggatagaat atcccagacc aactacattc caactcagca
    11341 agatgttctt cggacgagag tgaagactac aggcattgtg gagacccact tcaccttcaa
    11401 ggagctctac ttcaaaatgt ttgatgtagg tggccaaaga tccgaacgga aaaagtggat
    11461 ccactgtttt gagggagtga cagcaattat cttttgtgtg gctctcagtg attacgacct
    11521 tgttctggct gaggacgagg aaatgaaccg aatgcatgaa agcatgaaat tgtttgacag
    11581 catttgtaac aacaaatggt ttacagacac ttcaatcatt ctcttcctta ataagaaaga
    11641 cctttttgag gaaaaaataa agaggagtcc attaacaatc tgttatccag aatacacagg
    11701 ttccaatacg tacgaagagg cagctgctta catccagtgc cagtttgaag atctgaaccg
    11761 aagaaaggac accaagga
//
