LOCUS       sequence-405533-        2222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 47434 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-vapol
FEATURES             Location/Qualifiers
     source          1..2222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(873..1461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(953..972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1552..2222,1..121))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
       61 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
      121 tgcgaaacga tcctcatcct gtctcttgat cagatattga tcccctgcgc catcagatcc
      181 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
      241 ccccagctgg caattccggt tcgcttgcta agcttgcatg cctgcaagtc gactctagag
      301 gagcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
      361 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      421 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      481 ggctgcctgt atcgagtggt gattttgtgc cgagctcatt actcgcatcc attctcaggc
      541 tgtctcgtct cgtctcaggt ctcaggagtt gatgcgaaga ttcgatgacc ggacacattt
      601 tccagtcacg gctcagaaga atgtttactg ttagtcctgc gagtcaaatc ttgggccgga
      661 cgagcaacag cggatccccg cacaacaatt gaaaccggac gaatgcgtaa cgcaacacgg
      721 tacttccacc aatgcgagac caccaggata catagattac cacaactccg agcccttcca
      781 cccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      841 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      901 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      961 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1021 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1081 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1141 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1201 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1261 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1321 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1381 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1441 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1501 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt attatttgcc
     1561 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     1621 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     1681 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     1741 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     1801 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     1861 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     1921 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     1981 gataccagca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     2041 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcgtagaat
     2101 ctcactctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     2161 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     2221 gc
//
