LOCUS       sequence-405534-       11382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-SV40-Galpha-s ONE-GO
FEATURES             Location/Qualifiers
     source          1..11382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..221
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        33..229
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      80..215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(571..573,574..1284)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(613..634)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(874..893)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1221..1242
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2153..2727,2728..2730,2731..2739)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2320..2337)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2547..2566
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2750..2770
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             2879..3391
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             3401..3454
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5151..5739
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5204..5224)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5622..5633)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5946..6579
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6707..6723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6707..6723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6720..6742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6731..6747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6755..6761,6762..6779,6780..6785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6800..6821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6938..6955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7109..7697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7189..7208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7868..8659,8660..8728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8491..8510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8729..8833)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8881..9015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8918..8937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8972..8991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9183..9816
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9863..9988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10485..10718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10903..10947
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11210..11325
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
       61 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      121 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaac tcgagactag
      241 ttctagagcg gccgcggatc catgggctgc ctcgggaaca gtaagaccga ggaccagcgc
      301 aacgaggaga aggcgcagcg tgaggccaac aaaaagatcg agaagcagct gcagaaggac
      361 aagcaggtct accgggccac gcaccgcctg ctgctgctgg gtgctggaga atctggtaaa
      421 agcaccattg tgaagcagat gaggatcctg catgttaatg ggtttaatgg agacagtgag
      481 aaggcaacca aagtgcagga catcaaaaac aacctgaaag aggcgattga aaccattgtg
      541 gccgccatga gcaacctggg tggcggaggg gtgagcaagg gcgaggagct gttcaccggg
      601 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      661 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
      721 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
      781 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      841 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      901 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      961 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1021 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     1081 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1141 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac
     1201 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1261 ctcggcatgg acgagctgta caagggcggt gggggagtgc cccccgtgga gctggccaac
     1321 cccgagaacc agttcagagt ggactacatt ctgagtgtga tgaacgtgcc tgactttgac
     1381 ttccctcccg aattctatga gcatgccaag gctctgtggg aggatgaagg agtgcgtgcc
     1441 tgctacgaac gctccaacga gtaccagctg attgactgtg cccagtactt cctggacaag
     1501 atcgacgtga tcaagcaggc tgactatgtg ccgagcgatc aggacctgct tcgctgccgt
     1561 gtcctgactt ctggaatctt tgagaccaag ttccaggtgg acaaagtcaa cttccacatg
     1621 tttgacgtgg gtggccagcg cgatgaacgc cgcaagtgga tccagtgctt caacgatgtg
     1681 actgccatca tcttcgtggt ggccagcagc agctacaaca tggtcatccg ggaggacaac
     1741 cagaccaacc gcctgcagga ggctctgaac ctcttcaaga gcatctggaa caacagatgg
     1801 ctgcgcacca tctctgtgat cctgttcctc aacaagcaag atctgctcgc tgagaaagtc
     1861 cttgctggga aatcgaagat tgaggactac tttccagaat ttgctcgcta cactactcct
     1921 gaggatgcta ctcccgagcc cggagaggac ccacgcgtga cccgggccaa gtacttcatt
     1981 cgagatgagt ttctgaggat cagcactgcc agtggagatg ggcgtcacta ctgctaccct
     2041 catttcacct gcgctgtgga cactgagaac atccgccgtg tgttcaacga ctgccgtgac
     2101 atcattcagc gcatgcacct tcgtcagtac gagctgctat aagcggccgc cgcccctctc
     2161 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     2221 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     2281 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     2341 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     2401 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     2461 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     2521 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     2581 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     2641 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     2701 ttttcctttg aaaaacacga tgataagctt gccacaaccc gtaccaaaga tggggagtag
     2761 caagagcaag acgtcgaatt ccggaatcaa gcttggtgga tcatcaggag gtagcaggga
     2821 gctggcctgg ggcatcagcg agtggctgga ggagtggggc agcggaggtg gtggatccat
     2881 ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga
     2941 ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac
     3001 tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat
     3061 catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt
     3121 ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat
     3181 cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt
     3241 gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga
     3301 cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac
     3361 cggctggcgg ctgtgcgaac gcattctggc gggatcagga gaaggtagag gaagtctact
     3421 aacatgcggg gacgtggagg aaaatcccgg cccaatggat gaagagcgcg ccctctacat
     3481 cgtccgggcc ggcgaggcgg gggctatcga gcgggtcctg agggattaca gcgacaagca
     3541 tagggctact ttcaaatttg aatcagcaga tgaagataaa agaaagaaac tctgtgaagg
     3601 catatttaag gtccttgtca aggaggtccc aacgacatgc caagtgtcct gcctggaagt
     3661 actccgcatt ctctccagag acaaaaagat cttagttcct gtaacaacta aggaaaatat
     3721 gcaaatactg ctgagactag ccaagctcca cgagtcagac gattctttgg agaaggtgtc
     3781 agagttccca gttattgtgg aatcattgaa gtgtctgtgt aacatcgtgt tcaacagtca
     3841 gatggcacag cagcttagcc tggaactgaa tctcgctgca aagctctgta acctcctaag
     3901 gaagtgcaag gaccgaaaat ttatcaatga cataaagtgc tttgacttgc gcttgctctt
     3961 cgtcttgtca cttttgcaca ctgacatcag gtcacaattg cgctatgagc tccagggact
     4021 cccgctgcta acccagatct tggaaagtgc ctttagcatc aagtggaccg atgagtatga
     4081 gtcggccata gaccataatg gacctcctct ctcacctcag gagacagact gtgccattga
     4141 ggccctcaaa gctctgttca atgtgacggt agacagttgg aaggtgcata aagagagcga
     4201 ttctcatcag tttcgtgtca tggcagctgt ccttcgccat tgtttactaa ttgtaggtcc
     4261 aactgaagac aaaaccgaag agctgcacag taatgccgtc aaccttttaa gcaatgttcc
     4321 agtctcttgt ttggatgttc tcatttgtcc attaacccat gaagaaacag cccaagaggc
     4381 agcaactcta gatgaactgc ccagtgataa aacaacggag aaagacacag ctttgaaaaa
     4441 cagtaccatg gtatacaatg gcatgaatat ggaggccatt cacgttttac tcaattttat
     4501 ggagaagaga attgataagg gaagcagcta tagagagggt ctaactccag ttctcagctt
     4561 attaacagaa tgttcccgag cccatcggaa catccgaaaa tttctcaaag atcaggtttt
     4621 accgcctttg agagatgtga caaatcgacc agaagtcggc tcaaccgtga gaaataagct
     4681 ggttcgtctc atgacacatg ttgacctcgg agtcaagcag attgctgctg agttcctttt
     4741 tgtcctttgc aaagagagag tggatagcct gctgaaatac actggctatg ggaacgctgc
     4801 aggactcttg gcggccaggg gcctcttggc tggaggaaga ggagataatt ggtactcaga
     4861 ggacgaggac acggacactg aagaatacaa aaacgcaaaa ccaaacatta atcttatcac
     4921 tggtcattta gaggaaccaa tgccaaaccc tatagatgaa atgacagaag aacaaaaaga
     4981 atatgaagcc atgaaacttg tcaacatgct tgataaactt tccagagagg agttgcttag
     5041 accaatggga ctaaaacctg acgggacaat aacgcctttg gaggaagcac tcagccagta
     5101 ttctgtcatc gaagagacca gctcggacac agactgaacg cgtctggaac aatcaacctc
     5161 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5221 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5281 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5341 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5401 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5461 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5521 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5581 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5641 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5701 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     5761 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     5821 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     5881 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     5941 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     6001 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     6061 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     6121 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     6181 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     6241 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     6301 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     6361 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     6421 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6481 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6541 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6601 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     6661 gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6901 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8161 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8461 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8821 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9001 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     9061 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     9121 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     9181 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     9241 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     9301 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     9361 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9421 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     9481 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     9541 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     9601 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     9661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     9841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     9901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     9961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    10021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    10081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    10141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    10201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    10261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    10321 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    10381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    10441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    10501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    10561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    10621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    10681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    10741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    10801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    10861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    10921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    10981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    11041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    11101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    11161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa
    11221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    11281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    11341 ggacagcaga gatccagttt atcgatggtg tggaaagtcc cc
//
