LOCUS       sequence-405535-        2747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 51230 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-fcpF
FEATURES             Location/Qualifiers
     source          1..2747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(882..1470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(962..981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1561..2352)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctcagg agctaacagg
       61 attagtgcaa ttcgagttga atcactggga aaaacattgt cttctttttt atattatcat
      121 ttgcattagt gctgcagtcg tagatacttg ttggttgaaa gacatcagct gggagggact
      181 ggactagcgt ttggtaagga gacatacctg ttaacgttgg ttgcaaaatt ccatttcgcg
      241 atttatgtta tctgtaaatc ctgatttgtc tggaattctt gatacttccg tttttttaga
      301 ggccaatgat tagcatcggc gattctcaaa atagcatttt cgacatgcgg tgctgatttc
      361 ataaacatag acaacgcttt tacatgtaaa agtaacttgc ggacttggaa cagtgctctg
      421 tttttggtgt gaacgtaact cagcaatatt tctgtgctag caaggttttt tatgatcgac
      481 cgaagatctc aaaactccgg gtctttcaac tgtctgacta gaccatgttc gtaacgtcgg
      541 acagcagctt tcgttgtact ggtagaattt ctacgtgcga agcacgtgta ggcaggttga
      601 acgacgatcc ctgccgatgg atggattggc acgcggcgga acgctttcgt gatctacacc
      661 acctggatct tcacatatct tcgaaatcga aaaattaacc aagtcgacgg tatcgataat
      721 attctagctg agggtaccca atgcgagacc accaggatac atagattacc acaactccga
      781 gcccttccac ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      841 caaaaggcaa ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1321 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta
     1561 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     1621 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     1681 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     1741 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     1801 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     1861 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     1921 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     1981 tggctcgaag ataccagcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     2041 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     2101 gcggagaatc tcactctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     2161 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     2221 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     2281 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     2341 cgcttccctc atgcgaaacg atcctcatcc tgtctcttga tcagatattg atcccctgcg
     2401 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     2461 accagagggc gccccagctg gcaattccgg ttcgcttgct aagcttgcat gcctgcaagt
     2521 cgactctaga ggagcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     2581 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     2641 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     2701 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagct
//
