LOCUS       sequence-405536-        3566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 49202 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-P49
FEATURES             Location/Qualifiers
     source          1..3566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(141..160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(740..1531)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgcattct aggtgattat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      781 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      841 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      901 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      961 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1021 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1081 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1141 gatcaatgtc gatcgtggct ggctcgaaga taccagcaag aatgtcattg cgctgccatt
     1201 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1261 caatggtgac ttctacagcg cggagaatct cactctctcc aggggaagcc gaagtttcca
     1321 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     1381 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     1441 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     1501 tcgatacttc ggcgatcacc gcttccctca tgcgaaacga tcctcatcct gtctcttgat
     1561 cagatattga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     1621 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgcta
     1681 agcttgcatg cctgcaagtc gactctagag gagcggtgat cacaggcagc aacgctctgt
     1741 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     1801 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     1861 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     1921 cgagctcatt actcgcatcc attctcaggc tgtctcgtct cgtctcaggt ctcaggagtc
     1981 caaacggtac gtcagatccc acgtcactac cgcacccggt tattccagtg cgtaagctcg
     2041 tagtggaaga acaggctgcg gtagtgacgt ggaatttgac actttccaat cttcaagacc
     2101 tctagccagc aaagacttct tgaatctgca aggatattct atttagatat aagtatatac
     2161 ttgacagtga ggggggtcgt gaccgtctct cgctgggata gagaagatgg caaccattga
     2221 attgagaact cgaaacttga gcttggttgg atcttgatca ttctgaattt gcaggatagg
     2281 ttaacagtaa aagtcaatct tgcgctcgcc atctaccaga aagcacgtgg gtgcccagat
     2341 cctcccaacg agcgaacgga cggatgctag tcggggggat ctctgtgggc cattcaaacg
     2401 aaaggcagat ggcatcgttc caagatctat tggtcattgg gcttctcata cagtcattga
     2461 caccacgatt gaagacaaat ttttgttgac agtgaaatcg ttgcggacgc gctccgattc
     2521 aaaagtttcc ctactcggtc gcgtcaaccg accttcccct cgtgagttta cagcttgata
     2581 cgccctattt ggcttcctat gtaaagtctc cagtgatcat attaaagtca ttggtcatga
     2641 cactctcctt gttcaaaatt tggtaccata caatgcgcgg agcagtcagt tctcatttcc
     2701 agcgatcgtt aaaacaatta gagaaatgcc ttacttctct ctttatatct gtattactca
     2761 gagtcagtga gtagtacgaa tttccaacgt cctagctcca atttatggtt tcaaacaatc
     2821 cgaattcgtt ggtccacagt caaggaattc gcaagaatcc ctatttcact aggcgtgcac
     2881 tatggggtcg cttttgaggt tctcatttgc ggcgatcgtt aaaacgatta cagaaatgct
     2941 acacttcgct ctttatatat attactcatt gagcatcagg aatgtccaat gtcgtaactc
     3001 cgattcatgg tttcaaacaa tccgaattcg ttggtccgag gaatccgcaa aaagtaattg
     3061 tagtctatgt acgaccgagt cgctggtgag ggttttttgt cgttggattg ctcgagcgaa
     3121 ttctccgaga ttgtacagaa agctcaactt tcggaaggca tctatgtggt ccaaccaatc
     3181 tattcaaata tatcaattgt taagagcaga gttcgatcct cactatgtgg aggggcatgg
     3241 tgagattacg gagtgccgag acataagagc tccctgcaat caagttgatg gacatgcttc
     3301 gaaaatgcca tgactgagaa cgagaacgcc cagatgctcc gcggcacgag aactcgatga
     3361 acacgaaccg aatccaacgc caaaaacgcg ttgtccaaaa cattgcaact ctctctgata
     3421 aaaaccaaaa cgccgcaaat tgcaccagct tttttggttg tcctacgcct ctttccgcac
     3481 caccaatgcg agaccaccag gatacataga ttaccacaac tccgagccct tccacccaca
     3541 gaatcagggg ataacgcagg aaagaa
//
