LOCUS       sequence-405537-        2545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 25172 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-fcpB
FEATURES             Location/Qualifiers
     source          1..2545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      853..1441
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1342..1361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2516..2545,1..762)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
       61 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
      121 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
      181 ttggaaactt cggcttcccc tggagagagt gagattctcc gcgctgtaga agtcaccatt
      241 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
      301 gaatggcagc gcaatgacat tcttgctggt atcttcgagc cagccacgat cgacattgat
      361 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
      421 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
      481 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
      541 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
      601 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
      661 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
      721 cactacgtga aaggcgagat caccaaggta gtcggcaaat aataatcacc tagaatgcat
      781 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
      841 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      901 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      961 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1021 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1081 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1141 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1201 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1261 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1321 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1381 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1441 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1501 gttctttcct gcgttatccc ctgattctgt gggtggaagg gctcggagtt gtggtaatct
     1561 atgtatcctg gtggtctcgc attcttgact tctggcaacc gcgaaatatg cgctgtgtat
     1621 agtaaggaag ccgcagctgg ggcgagcgtc gtagcgggag gcccccaaaa ggatacgtgt
     1681 tcaaccagga tttgctgttc acggagttga tgtcaggatg tcgctcttgt gtcttatcct
     1741 gctgtattta atagattgaa tttcgaacct ctacgggcca aagattactg tcagttgact
     1801 gtgacagtga agacgacgct gaaggtatgt ttcgagaaaa ctcgccccct gcctagcttg
     1861 cacgacgctg aagctatgtg tcttgagaaa actcaccctg tgccttgctc agtttctgct
     1921 agtccacgcc aacgatacgg cacgccgtgc cctattcgga cattctttct ctttccaaaa
     1981 gttctttgct tgccagcgtt gaatgttgaa cttgtgtaca ccgtgaatgg gcgagattgg
     2041 cattcacgct gtagacgagc tagtgttact cctgagacct gagacgagac gagacagcct
     2101 gagaatggat gcgagtaatg agctcggcac aaaatcacca ctcgatacag gcagcccatc
     2161 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     2221 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     2281 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca ccgctcctct
     2341 agagtcgact tgcaggcatg caagcttagc aagcgaaccg gaattgccag ctggggcgcc
     2401 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     2461 ctgatggcgc aggggatcaa tatctgatca agagacagga tgaggatcgt ttcgcatgag
     2521 ggaagcggtg atcgccgaag tatcg
//
