LOCUS       sequence-405538-        3346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 34971 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-H4
FEATURES             Location/Qualifiers
     source          1..3346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1317..2108
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2199..2787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2688..2707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcttaaatac aataacggtc atctatttag ctgtgcgcta cttagcaaac cgagacatta
       61 tttaccccgc acgcgttgga cgagcgtaac gcacgacaag gccgtggacg ttcttggtag
      121 tagtagtaat atgttgttgg cgcggaggag tgtacccgtt tcccgggaaa gagtccgacg
      181 gcagggcatc ggctgccgca gctcccgttt tctccgcctc gctcgatgcg ttcgtgacga
      241 cgctaacgat ggacgaccaa ctttagtcgt tccccttgta gtgaggattt gcagaagact
      301 tgtctactat tagtgttcgt gtgtgcataa tctttgattg ttgtaccggg tcgggtttca
      361 cagaaattgc ttggcagtca ctgttagttt gacgagggct tccttcaaac gagtgcagca
      421 tcttgataag tcgatagggt ttgctgacgg ggactttgtt ctctcccgtg taatcggaag
      481 cagcaaccga atttcgtcta catattcgta gtcgcatggg gcaagaagct tcactcccgg
      541 cgggagctac accggaagac tatgaggaac accagcgcac ggcctcggca ccagcgactc
      601 acgcaaaacg ccccgggtcg cgaaggatgg gagccgtctt tcaacgggga tccggcaatg
      661 cccagaacgg taaccacaat cacgggaact ccacgttaga agactttgaa gggcgagaag
      721 cggcacgcgc tgctgcttcg gggggacatt tctaccctcc cgacgatgct tccgaaacca
      781 gctttaccac ggcgtcgcag gcattgccgc cttccggaac cgcaaccacc acagcaacac
      841 accagcagca tcagcagtac gccaacaatc tcctgagacc tgagacgaga cgagacagcc
      901 tgagaatgga tgcgagtaat gagctcggca caaaatcacc actcgataca ggcagcccat
      961 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     1021 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     1081 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgctcctc
     1141 tagagtcgac ttgcaggcat gcaagcttag caagcgaacc ggaattgcca gctggggcgc
     1201 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
     1261 tctgatggcg caggggatca atatctgatc aagagacagg atgaggatcg tttcgcatga
     1321 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1381 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     1441 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     1501 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagtgaga
     1561 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1621 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gctggtatct
     1681 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1741 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1801 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     1861 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     1921 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     1981 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     2041 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     2101 gcaaataata atcacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2161 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2221 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2281 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2341 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2401 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2461 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2521 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2581 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2641 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2701 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2761 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2821 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtgggt
     2881 ggaagggctc ggagttgtgg taatctatgt atcctggtgg tctcgcattg ttggctgttg
     2941 tttgttttcg gtattgagta aaacgattgt cggttactgc cgagagcgaa ttgtgagagt
     3001 gtaaccttca cagcgcttcg aaagccggga gtcaccgctg ctcgatggcc agtactagtt
     3061 gctgttcgta tggttctaca tacatagtgt tccgtaagct tcttacaatg tacgatgttg
     3121 gcgcattttg ttggagctgt cagtttgtcc tcgcgctacg aatgggtgtg taatagtcat
     3181 gcttatttgg attggaaatg gttttctgtc tgtgaaagat ctgcccaatg tcggttcgcg
     3241 tgtcccggtt gactactttg ggcgaacgct tagggttgac gggttaaccg ttagtacaag
     3301 ggaatttcag tttgacgcaa agtacaatgc aatcaatatt gaatga
//
