LOCUS       sequence-405539-        2746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 54983 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-NR
FEATURES             Location/Qualifiers
     source          1..2746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1100..1688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1180..1199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1779..2570)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaggagcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
       61 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
      121 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
      181 atgggctgcc tgtatcgagt ggtgattttg tgccgagctc attactcgca tccattctca
      241 ggctgtctcg tctcgtctca ggtctcagga gagcgaacag aagacccaat cgtggagcag
      301 tcatcatcat tgtggaaatg tgtcctagac tgttgcagac agtcaccaac ggaatgcttt
      361 cgttaagagc aaggcgacaa cggttccttc gttgagatgg tggttccact gtgcaggtca
      421 cgtagtgaag tgatgttgcg gtagtctgca atcccgctag atacacgtca ttcgtgaaag
      481 gcctgttgtt ttcatgtccg ggtctgcacg ggcactagaa ggaaggaagc aaagacgaac
      541 ggaagtgact gtaaacgaga agtgcacgaa gccttttctt gtgacgtcac aacccgaata
      601 gccctacgtg gcgtgcgact ttccggcact ttggatatcg ttgccctata tgtattgggt
      661 gtatacatgt cgtattccaa ctacgacaga aaacagtcag ttacgggtat tcgaacttcc
      721 ggcacctgca gcgaggagtt ttgtgtccgt cgcacatcct ccgtgctttc ggcacctacg
      781 taggcggcaa acttcctgcc ttccgccccc gccttgcggc attccggctc cgggccagaa
      841 ttgcccgggt gttcacaatt ttgcctcctc acgaaaaaac gttctacttt gtattttggt
      901 cgggtttcgg atccttccag caaccattct cattcaaagt caccacttgt gcgaacgaat
      961 gcgagaccac caggatacat agattaccac aactccgagc ccttccaccc acagaatcag
     1021 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1081 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1141 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1201 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1261 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1321 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1381 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1441 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1501 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1561 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1621 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1681 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1741 cacgttaagg gattttggtc atgcattcta ggtgattatt atttgccgac taccttggtg
     1801 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     1861 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     1921 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     1981 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     2041 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     2101 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     2161 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat accagcaaga
     2221 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     2281 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc actctctcca
     2341 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     2401 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     2461 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     2521 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat gcgaaacgat
     2581 cctcatcctg tctcttgatc agatattgat cccctgcgcc atcagatcct tggcggcaag
     2641 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
     2701 aattccggtt cgcttgctaa gcttgcatgc ctgcaagtcg actcta
//
