LOCUS       sequence-405541-        3047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 23658 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-Fld
FEATURES             Location/Qualifiers
     source          1..3047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1339..2130
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2221..2809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2710..2729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactgctacg gtactttaat atgcaaattc gagatggaac catcgcatcc ctcatgctag
       61 aacggacaca cgttggcacg tgtcaaggaa actcatgggc gtggcattct tcagacacac
      121 cgacattgaa tgagagcgta ggaccaatag tggggttgct gtttgttccc aagtaccggt
      181 cccgctctca ggagggaggt agcgccttat gtcagcgcaa tcctttgtat cctgagaaaa
      241 agggcctaac acaagcttct tccctacaag gacagtagat atccactagt cgcctttggt
      301 ttgagtaact ctaaacagac tactgtagaa gtcctatccg tcagctcgcc cctaacggtg
      361 aaagccagca gaacggcgat tgcgattggt tgtaatgcac aggaggcgca ctcctagacc
      421 gtgctcaaca cacgcattgc acacgcacgg caatccaacc ctgactggac gaagggtggg
      481 gttcacatca aggaattctt gattgtgtta cagttcgtgt tgctggacgt ctctcaaact
      541 atcagtcttt ctgtctgtcc gcccacctta catcgagcta tcctgcgtcc attatcactc
      601 cccatagcac gtccaaataa tagtgtttcc aacggcagaa actccatgct tacctatgca
      661 ttggttggtt cttacttatg gttgcaacat aaatgtatgg agaggagact ttgaatttgg
      721 agcttgatct ccaccaacgt gcgattccgt cgatagaact atcctctggt acaaacgatt
      781 gctagagagc gaatcaatct gaactaaaaa tagttagtat agtaggaagc ttatgcgatg
      841 gctggttccg gtgttttcgc ttcgtcctcg aggagtccac attcaacgac actcctgaga
      901 cctgagacga gacgagacag cctgagaatg gatgcgagta atgagctcgg cacaaaatca
      961 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     1021 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     1081 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     1141 ctgcctgtga tcaccgctcc tctagagtcg acttgcaggc atgcaagctt agcaagcgaa
     1201 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     1261 atggctttct tgccgccaag gatctgatgg cgcaggggat caatatctga tcaagagaca
     1321 ggatgaggat cgtttcgcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     1381 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     1441 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     1501 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     1561 gcttcccctg gagagagtga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     1621 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     1681 aatgacattc ttgctggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     1741 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     1801 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     1861 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1921 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1981 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     2041 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     2101 ggcgagatca ccaaggtagt cggcaaataa taatcaccta gaatgcatga ccaaaatccc
     2161 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2221 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2281 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2341 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2401 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2461 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2521 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2581 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2641 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2701 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2761 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2821 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2881 gttatcccct gattctgtgg gtggaagggc tcggagttgt ggtaatctat gtatcctggt
     2941 ggtctcgcat tttcacgttc tcgattatcg tgtatatgaa tgaattggcg gtgtttggaa
     3001 gatattgtgt gctgggtgaa agaatttccg atggaacgag tattacg
//
