LOCUS       sequence-405569-        2069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a small non-coding spacer sequence with 
            AF overhangs used to build level 1 constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AF-spacer
FEATURES             Location/Qualifiers
     source          1..2069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             688..1479
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(1570..2069,1..89)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(2059..2069,1..9)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
       61 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      181 gtggaagggc tcggagttgt ggtaatctat gtatcctggt ggtctcgagc gcaatttgtg
      241 ctccggagac ctgagacgag acgagacagc ctgagaatgg atgcgagtaa tgagctcggc
      301 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
      361 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
      421 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
      481 agagcgttgc tgcctgtgat caccgctcct ctagagtcga cttgcaggca tgcaagctta
      541 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
      601 gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc aatatctgat
      661 caagagacag gatgaggatc gtttcgcatg agggaagcgg tgatcgccga agtatcgact
      721 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
      781 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
      841 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
      901 gaaacttcgg cttcccctgg agagagtgag attctccgcg ctgtagaagt caccattgtt
      961 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     1021 tggcagcgca atgacattct tgctggtatc ttcgagccag ccacgatcga cattgatctg
     1081 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     1141 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     1201 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     1261 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     1321 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     1381 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     1441 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat aatcacctag aatgcatgac
     1501 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1561 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1621 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1681 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1741 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1801 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1981 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2041 cacgagggag cttccagggg gaaacgcct
//
