LOCUS       sequence-405570-        2161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a kozak consensus sequence and a 
            chloroplast signal peptide sequence with BC overhangs used to build 
            level 1 constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_BC-chl_sig_pep
FEATURES             Location/Qualifiers
     source          1..2161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      365..953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     854..873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1644..2161,1..274)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
       61 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
      121 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
      181 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
      241 gaaaggcgag atcaccaagg tagtcggcaa ataataatca cctagaatgc atgaccaaaa
      301 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      361 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      421 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      481 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      541 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      601 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      661 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      721 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      781 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      961 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1021 ctgcgttatc ccctgattct gtgggtggaa gggctcggag ttgtggtaat ctatgtatcc
     1081 tggtggtctc gcatttgcac attcgcagtc ttcacagtga aggaagttgg ctgtgtggtg
     1141 aaggcagatg ccacagccaa aagggctgcg atgcaaaagg atctcatggt ggccccagta
     1201 tgagacctga gacgagacga gacagcctga gaatggatgc gagtaatgag ctcggcacaa
     1261 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     1321 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     1381 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     1441 cgttgctgcc tgtgatcacc gctcctctag agtcgacttg caggcatgca agcttagcaa
     1501 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     1561 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaata tctgatcaag
     1621 agacaggatg aggatcgttt cgcatgaggg aagcggtgat cgccgaagta tcgactcaac
     1681 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1741 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     1801 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1861 cttcggcttc ccctggagag agtgagattc tccgcgctgt agaagtcacc attgttgtgc
     1921 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1981 agcgcaatga cattcttgct ggtatcttcg agccagccac gatcgacatt gatctggcta
     2041 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2101 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2161 g
//
