LOCUS       sequence-405572-        2125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a kozak consensus sequence and a 
            secretion signal peptide sequence with BC overhangs used to build 
            level 1 constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_BC-sec_sig_pep
FEATURES             Location/Qualifiers
     source          1..2125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      771..1359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1260..1279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2014..2125,1..680)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
       61 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
      121 gagagagtga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
      181 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
      241 ttgctggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
      301 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
      361 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
      421 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
      481 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
      541 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
      601 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
      661 ccaaggtagt cggcaaataa taatcaccta gaatgcatga ccaaaatccc ttaacgtgag
      721 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      781 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      841 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      901 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      961 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1021 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1081 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1141 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1201 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1261 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1321 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1381 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1441 gattctgtgg gtggaagggc tcggagttgt ggtaatctat gtatcctggt ggtctcgcat
     1501 ttgagcccca gccgagaagc ttgcgaggag aaacggaagg atacaacgaa gattcatggt
     1561 ggccccagta ggagacctga gacgagacga gacagcctga gaatggatgc gagtaatgag
     1621 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     1681 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     1741 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     1801 gatgacagag cgttgctgcc tgtgatcacc gctcctctag agtcgacttg caggcatgca
     1861 agcttagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     1921 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaata
     1981 tctgatcaag agacaggatg aggatcgttt cgcatgaggg aagcggtgat cgccgaagta
     2041 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     2101 gccgtacatt tgtacggctc cgcag
//
