LOCUS       sequence-405573-        2170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a kozak consensus sequence and a 
            mitochondria signal peptide sequence with BC overhangs used to build
            level 1 constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_BC-mito_sig_pep
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      268..856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     757..776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1556..2170,1..177)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
       61 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
      121 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataataa
      181 tcacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      241 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      301 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      361 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      421 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      481 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      541 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      601 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      661 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      721 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      781 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      841 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      901 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtgggtg gaagggctcg
      961 gagttgtggt aatctatgta tcctggtggt ctcgcatttg ttccccaatg gcgtggctaa
     1021 gcttgaggag catcgagagc ggttgcccga tgcctccggc agctccgagc acggcgacct
     1081 ttttcgacgc gctgctcatg gtggccccag tatgagacct gagacgagac gagacagcct
     1141 gagaatggat gcgagtaatg agctcggcac aaaatcacca ctcgatacag gcagcccatc
     1201 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     1261 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     1321 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca ccgctcctct
     1381 agagtcgact tgcaggcatg caagcttagc aagcgaaccg gaattgccag ctggggcgcc
     1441 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     1501 ctgatggcgc aggggatcaa tatctgatca agagacagga tgaggatcgt ttcgcatgag
     1561 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     1621 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     1681 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     1741 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagtgagat
     1801 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     1861 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg ctggtatctt
     1921 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     1981 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     2041 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     2101 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     2161 cgcgccgaag
//
