LOCUS       sequence-405581-        9922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-i3 G183S ONE-GO
FEATURES             Location/Qualifiers
     source          1..9922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    189..304
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        362..665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        666..869
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     820..840
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     866..890
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1344..1346,1347..1349,1350..2060)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1389..1410)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1650..1669)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1997..2018
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2800..3374,3375..3377,3378..3386)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2967..2984)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3194..3213
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3397..3417
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3523..4038
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4052..4640
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4105..4125)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4523..4534)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4847..5480
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5608..5624)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5608..5624)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5621..5643)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5632..5648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5656..5662,5663..5680,5681..5686))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5701..5722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5839..5856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6010..6598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6090..6109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6769..7560,7561..7629))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7392..7411
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7630..7734)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7782..7916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7819..7838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7873..7892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8084..8717
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8764..8889
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9386..9619
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9804..9848
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
       61 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
      121 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
      181 gtatcgcctt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      241 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      301 attttcgggt ttattacagg gacagcagag atccagttta tcgataagct tgggagttcc
      361 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      421 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      481 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      541 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      601 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      661 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      721 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      781 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      841 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      901 gccatccacg ctgttttgac ctccatagaa gacaccgact ctactagagg atctatttcc
      961 ggtgaattcc tcgagactag ttctagagcg gccgcggatc catgggctgc acgttgagcg
     1021 ccgaggacaa ggcggcggtg gagcggagta agatgatcga ccgcaacttg cgggaggacg
     1081 gagagaaagc ggccaaagaa gtgaagctgc tgctgctcgg cgctggagaa tctggtaaaa
     1141 gtactattgt gaaacagatg aaaatcattc atgaggatgg ctattccgag gacgagtgta
     1201 agcagtataa agttgtcgtc tacagcaata ccattcagtc catcattgca atcataagag
     1261 ccatgggacg gttgaagatt gattttgggg aagctgccag agcggatgat gcccgacagt
     1321 tatttgtttt agctggcagc gctatggtga gcaagggcga ggagctgttc accggggtgg
     1381 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1441 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1501 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg
     1561 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1621 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1681 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1741 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1801 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1861 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1921 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     1981 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2041 gcatggacga gctgtacaag agcgctgagg aaggagtcat gacttcagaa ctagcaggcg
     2101 tgattaaacg tttatggcga gatggcgggg tgcaggcatg cttcagcagg tccagggaat
     2161 atcagctcaa tgattctgct tcatattacc taaatgattt ggatagaata tcccagacca
     2221 actacattcc aactcagcaa gatgttcttc ggacgagagt gaagactaca agcattgtgg
     2281 agacccactt caccttcaag gagctctact tcaaaatgtt tgatgtaggt ggccaaagat
     2341 ccgaacggaa aaagtggatc cactgttttg agggagtgac agcaattatc ttttgtgtgg
     2401 ctctcagtga ttacgacctt gttctggctg aggacgagga aatgaaccga atgcatgaaa
     2461 gcatgaaatt gtttgacagc atttgtaaca acaaatggtt tacagacact tcaatcattc
     2521 tcttccttaa taagaaagac ctttttgagg aaaaaataaa gaggagtcca ttaacaatct
     2581 gttatccaga atacacaggt tccaatacgt acgaagaggc agctgcttac atccagtgcc
     2641 agtttgaaga tctgaaccga agaaaggaca ccaaggaggt ctacactcac tttacctgtg
     2701 ccactgacac caaaaacgtg cagtttgttt ttgatgctgt tacagatgtc atcattaaaa
     2761 acaacttaaa ggaatgtggg ctttactgag cggccgccgc ccctctccct cccccccccc
     2821 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     2881 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     2941 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3001 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3061 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3121 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3181 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3241 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3301 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     3361 aacacgatga taagcttgcc acaacccgta ccaaagatgg ggagtagcaa gagcaagacg
     3421 tcgaattccg gaatcaagct tggtggatct tctcgaggtt actaccatgg tatttgggtg
     3481 ggtgaagaag gtcgactttc tcgaggtggc accggtggat ccatggtctt cacactcgaa
     3541 gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt ccttgaacag
     3601 ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat ccaaaggatt
     3661 gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc gtatgaaggt
     3721 ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta ccctgtggat
     3781 gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg ggttacgccg
     3841 aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag
     3901 atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg cctgatcaac
     3961 cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg gcggctgtgc
     4021 gaacgcattc tggcgtaaac gcgtctggaa caatcaacct ctggattaca aaatttgtga
     4081 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4141 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4201 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4261 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4321 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4381 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4441 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4501 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4561 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4621 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     4681 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4741 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     4801 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     4861 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     4921 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     4981 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     5041 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     5101 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     5161 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     5221 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     5281 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5341 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5401 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5461 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     5521 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac
     5581 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5641 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5701 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5761 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5821 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5881 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5941 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6001 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6061 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6121 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6181 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6241 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6301 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6361 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6421 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6481 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6541 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6601 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6661 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6721 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6781 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6841 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6901 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6961 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7021 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7081 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7141 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7201 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7261 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7321 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7381 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7441 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7501 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7561 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7621 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7681 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7741 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
     7801 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7861 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     7921 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     7981 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     8041 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
     8101 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     8161 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     8221 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     8281 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     8341 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     8401 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     8461 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     8521 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8581 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8641 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8701 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8761 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8821 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8881 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8941 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     9001 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     9061 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     9121 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     9181 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     9241 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9301 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9361 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9421 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9481 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9541 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9601 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9661 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9721 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9781 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9841 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9901 tgatagtagg aggcttggta gg
//
