LOCUS       sequence-405583-        2170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a kozak consensus sequence and a ER 
            signal peptide sequence with BC overhangs used to build level 1 
            constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_BC-ER_sig_pep
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(828..1416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(908..927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1507..2170,1..128))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
       61 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
      121 tccctcatgc gaaacgatcc tcatcctgtc tcttgatcag atattgatcc cctgcgccat
      181 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
      241 gagggcgccc cagctggcaa ttccggttcg cttgctaagc ttgcatgcct gcaagtcgac
      301 tctagaggag cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
      361 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
      421 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
      481 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctcattact cgcatccatt
      541 ctcaggctgt ctcgtctcgt ctcaggtctc atactggggc caccatgata ttcatgagaa
      601 ttgccgtagc agcactggcc ttgctggctg ctccctccat tcgtgccgaa gaggccggtg
      661 aagaggccaa gatgggtacc gtgcaaatgc gagaccacca ggatacatag attaccacaa
      721 ctccgagccc ttccacccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      781 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      841 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      901 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      961 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1021 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1081 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1141 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1201 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1261 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1321 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1381 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1441 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
     1501 tgattattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc
     1561 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
     1621 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
     1681 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
     1741 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
     1801 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
     1861 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
     1921 cgtggctggc tcgaagatac cagcaagaat gtcattgcgc tgccattctc caaattgcag
     1981 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
     2041 tacagcgcgg agaatctcac tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
     2101 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
     2161 actgtgtggc
//
