LOCUS       sequence-405591-        9543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-SV40-Galpha-i3 ONE-GO
FEATURES             Location/Qualifiers
     source          1..9543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    189..304
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        372..582
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        394..590
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      441..576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(965..967,968..970,971..1681)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1010..1031)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1271..1290)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1618..1639
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2421..2995,2996..2998,2999..3007)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2588..2605)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2815..2834
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3018..3038
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3144..3659
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    3673..4261
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3726..3746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4144..4155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4468..5101
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5229..5245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5229..5245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5242..5264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5253..5269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5277..5283,5284..5301,5302..5307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5322..5343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5460..5477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5631..6219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5711..5730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6390..7181,7182..7250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7013..7032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7251..7355)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7403..7537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7440..7459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7494..7513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7705..8338
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8385..8510
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9007..9240
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9425..9469
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
       61 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
      121 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
      181 gtatcgcctt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      241 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      301 attttcgggt ttattacagg gacagcagag atccagttta tcgatggtgt ggaaagtccc
      361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      421 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      481 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      541 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa ctcgagacta
      601 gttctagagc ggccgcggat ccatgggctg cacgttgagc gccgaggaca aggcggcggt
      661 ggagcggagt aagatgatcg accgcaactt gcgggaggac ggagagaaag cggccaaaga
      721 agtgaagctg ctgctgctcg gcgctggaga atctggtaaa agtactattg tgaaacagat
      781 gaaaatcatt catgaggatg gctattccga ggacgagtgt aagcagtata aagttgtcgt
      841 ctacagcaat accattcagt ccatcattgc aatcataaga gccatgggac ggttgaagat
      901 tgattttggg gaagctgcca gagcggatga tgcccgacag ttatttgttt tagctggcag
      961 cgctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1021 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1081 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1141 caccctcgtg accaccttcg gctacggcct gatgtgcttc gcccgctacc ccgaccacat
     1201 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1261 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1321 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1381 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1441 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1501 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1561 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1621 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1681 gagcgctgag gaaggagtca tgacttcaga actagcaggc gtgattaaac gtttatggcg
     1741 agatggcggg gtgcaggcat gcttcagcag gtccagggaa tatcagctca atgattctgc
     1801 ttcatattac ctaaatgatt tggatagaat atcccagacc aactacattc caactcagca
     1861 agatgttctt cggacgagag tgaagactac aggcattgtg gagacccact tcaccttcaa
     1921 ggagctctac ttcaaaatgt ttgatgtagg tggccaaaga tccgaacgga aaaagtggat
     1981 ccactgtttt gagggagtga cagcaattat cttttgtgtg gctctcagtg attacgacct
     2041 tgttctggct gaggacgagg aaatgaaccg aatgcatgaa agcatgaaat tgtttgacag
     2101 catttgtaac aacaaatggt ttacagacac ttcaatcatt ctcttcctta ataagaaaga
     2161 cctttttgag gaaaaaataa agaggagtcc attaacaatc tgttatccag aatacacagg
     2221 ttccaatacg tacgaagagg cagctgctta catccagtgc cagtttgaag atctgaaccg
     2281 aagaaaggac accaaggagg tctacactca ctttacctgt gccactgaca ccaaaaacgt
     2341 gcagtttgtt tttgatgctg ttacagatgt catcattaaa aacaacttaa aggaatgtgg
     2401 gctttactga gcggccgccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     2461 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     2521 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     2581 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     2641 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     2701 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     2761 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     2821 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     2881 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     2941 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     3001 cacaacccgt accaaagatg gggagtagca agagcaagac gtcgaattcc ggaatcaagc
     3061 ttggtggatc ttctcgaggt tactaccatg gtatttgggt gggtgaagaa ggtcgacttt
     3121 ctcgaggtgg caccggtgga tccatggtct tcacactcga agatttcgtt ggggactggc
     3181 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     3241 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     3301 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     3361 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     3421 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     3481 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     3541 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     3601 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaaa
     3661 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3721 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3781 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3841 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3901 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3961 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4021 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     4081 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4141 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4201 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4261 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     4321 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     4381 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     4441 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     4501 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     4561 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     4621 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     4681 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     4741 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     4801 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     4861 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     4921 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4981 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5041 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5101 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     5161 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg
     5221 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5281 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5341 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5401 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5461 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5521 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5581 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6061 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7321 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7381 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
     7441 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7501 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     7561 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     7621 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     7681 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     7741 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     7801 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7861 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     7921 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     7981 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     8041 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     8101 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     8161 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8221 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8281 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8341 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8401 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8461 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8521 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8581 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8641 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8701 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8761 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8821 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     8881 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8941 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9001 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9061 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9121 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9181 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9241 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9301 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9361 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9421 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9481 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9541 agg
//
