LOCUS       sequence-405594-        2776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a green lantern fluorescent protein with 
            CD overhangs used to build a level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_CD-greenlantern
FEATURES             Location/Qualifiers
     source          1..2776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2..793)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             join(1238..1240,1241..1243,1244..1954)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(1283..1304)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     rep_origin      complement(2099..2687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2179..2198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
       61 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
      121 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
      181 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
      241 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
      301 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
      361 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
      421 ctggctcgaa gataccagca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
      481 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
      541 cgcggagaat ctcactctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
      601 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
      661 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
      721 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
      781 ccgcttccct catgcgaaac gatcctcatc ctgtctcttg atcagatatt gatcccctgc
      841 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
      901 taccagaggg cgccccagct ggcaattccg gttcgcttgc taagcttgca tgcctgcaag
      961 tcgactctag aggagcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat
     1021 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
     1081 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
     1141 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctca ttactcgcat
     1201 ccattctcag gctgtctcgt ctcgtctcag gtctcaaatg gtgagcaagg gcgaggagct
     1261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1321 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     1381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct taggctacgg
     1441 cgtggcctgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1501 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     1561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg tgctgaaggg
     1621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     1681 ccacaaggtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagac
     1741 ccgccacaac gttgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     1801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccaaact
     1861 gagcaaagac cccaacgaga agcgcgatca catggtcctg aaggagaggg tgaccgccgc
     1921 cgggattaca catgacatgg acgagctgta caagtcaggt cgagaccacc aggatacata
     1981 gattaccaca actccgagcc cttccaccca cagaatcagg ggataacgca ggaaagaaca
     2041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2521 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2761 tgcattctag gtgatt
//
