LOCUS       sequence-405596-        2767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a mCherry fluorescent protein with CD 
            overhangs used to build a level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_CD-mcherry
FEATURES             Location/Qualifiers
     source          1..2767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      819..1407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1308..1327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1552..2259)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1827..1846)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2096..2114
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             join(2704..2767,1..728)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
       61 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      121 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagtgaga
      181 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
      241 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gctggtatct
      301 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
      361 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
      421 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
      481 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
      541 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
      601 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
      661 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
      721 gcaaataata atcacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
      781 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      841 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      901 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      961 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1021 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1081 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1141 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1201 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1261 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1321 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1381 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1441 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtgggt
     1501 ggaagggctc ggagttgtgg taatctatgt atcctggtgg tctcgacctg acttgtacag
     1561 ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt
     1621 gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag
     1681 ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc
     1741 cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg
     1801 ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
     1861 gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg
     1921 ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
     1981 ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga
     2041 gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt
     2101 caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat
     2161 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
     2221 gatgatggcc atgttatcct cctcgccctt gctcaccatt tgagacctga gacaagacga
     2281 gacagcctga gaatggatgc gagtaatgag ctcggcacaa aatcaccact cgatacaggc
     2341 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2401 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2461 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2521 gctcctctag agtcgacttg caggcatgca agcttagcaa gcgaaccgga attgccagct
     2581 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     2641 ccaaggatct gatggcgcag gggatcaata tctgatcaag agacaggatg aggatcgttt
     2701 cgcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     2761 atcgagc
//
