LOCUS       sequence-405597-        2716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing an eYGFP-uv fluorescent protein with CD 
            overhangs used to build a level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_CD-eYGFP-uv
FEATURES             Location/Qualifiers
     source          1..2716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1873..2461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1953..1972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2552..2716,1..627))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
       61 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
      121 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
      181 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
      241 aatgtcgatc gtggctggct cgaagatacc agcaagaatg tcattgcgct gccattctcc
      301 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
      361 ggtgacttct acagcgcgga gaatctcact ctctccaggg gaagccgaag tttccaaaag
      421 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
      481 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
      541 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
      601 tacttcggcg atcaccgctt ccctcatgcg aaacgatcct catcctgtct cttgatcaga
      661 tattgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
      721 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctaagct
      781 tgcatgcctg caagtcgact ctagaggagc ggtgatcaca ggcagcaacg ctctgtcatc
      841 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
      901 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
      961 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
     1021 ctcattactc gcatccattc tcaggctgtc tcgtctcgtc tcaggtctca aatgacaaca
     1081 ttcaagattg agtcccgcat ccacggaaac cttaacggcg aaaaatttga attggtcggc
     1141 gggggcgttg gtgaagaggg tcgcttagaa attgagatga agacgaaaga caagccatta
     1201 gcattctcac cattcttatt aactacttgc atggggtacg gattttacca tttcgcctca
     1261 ttcccaaaag gtatcaagaa catttatctt catgccgcta cgaatggcgg atacacgaac
     1321 acccgtaagg aaatttacga agatggagga attcttgaag ttaattttcg ctacacatat
     1381 gagttcaata agatcatcgg ggatgtggaa tgcatcggac atggttttcc aagccagtcg
     1441 ccaattttca aagacacgat tgtcaagagt tgtccaaccg ttgatctgat gctgcctatg
     1501 agtggcaaca tcatcgcatc gtcttatgca tatgccttcc agttaaaaga tggtagcttt
     1561 tataccgcag aagtcaagaa caacattgat tttaaaaatc caatccacga atcgttcagc
     1621 aaatcaggac ctatgtttac acaccgccgt gtagaggaaa cattaacgaa ggagaatctg
     1681 gcgattgtcg agtatcagca agtgttcaat agcgctcctc gtgatatgtc aggtcgagac
     1741 caccaggata catagattac cacaactccg agcccttcca cccacagaat caggggataa
     1801 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2281 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2521 agggattttg gtcatgcatt ctaggtgatt attatttgcc gactaccttg gtgatctcgc
     2581 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     2641 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     2701 gctccattgc ccagtc
//
