LOCUS       sequence-405599-        2734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a mRFP1 fluorescent protein with CD 
            overhangs used to build a level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_CD-mRFP1
FEATURES             Location/Qualifiers
     source          1..2734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             565..1239
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     rep_origin      complement(1384..1972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1464..1483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2063..2734,1..120))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
       61 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
      121 gcgaaacgat cctcatcctg tctcttgatc agatattgat cccctgcgcc atcagatcct
      181 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
      241 cccagctggc aattccggtt cgcttgctaa gcttgcatgc ctgcaagtcg actctagagg
      301 agcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
      361 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
      421 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
      481 gctgcctgta tcgagtggtg attttgtgcc gagctcatta ctcgcatcca ttctcaggct
      541 gtctcgtctc gtctcaggtc tcaaatggct tcctccgaag acgttatcaa agagttcatg
      601 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      661 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      721 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      781 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
      841 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
      901 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
      961 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
     1021 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
     1081 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
     1141 tacaaaaccg acatcaaact ggacataacc tcccacaacg aagactacac catcgttgaa
     1201 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt caggtcgaga ccaccaggat
     1261 acatagatta ccacaactcc gagcccttcc acccacagaa tcaggggata acgcaggaaa
     1321 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1381 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1441 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1501 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1561 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1621 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1681 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1741 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1801 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1861 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1921 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1981 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2041 ggtcatgcat tctaggtgat tattatttgc cgactacctt ggtgatctcg cctttcacgt
     2101 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     2161 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
     2221 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
     2281 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     2341 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     2401 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     2461 ccagatcaat gtcgatcgtg gctggctcga agataccagc aagaatgtca ttgcgctgcc
     2521 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
     2581 caacaatggt gacttctaca gcgcggagaa tctcactctc tccaggggaa gccgaagttt
     2641 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
     2701 ccagcaaatc aatatcactg tgtggcttca ggcc
//
