LOCUS       sequence-405602-        9779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-hSyn-Galpha-oA ONE-GO
FEATURES             Location/Qualifiers
     source          1..9779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..87
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(991..1701,1702..1704,1705..1707))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1033..1054)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1382..1401
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1641..1662
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(2118..2565)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    2607..2722
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(2985..3029)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3214..3447
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3944..4069
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4116..4749
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4917..5051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4941..4960)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4995..5014
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5099..5203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5204..5272,5273..6064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5422..5441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6235..6823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6724..6743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6977..6994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7111..7132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7147..7152,7153..7170,7171..7177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7185..7201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7190..7212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7209..7225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7209..7225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7353..7986
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8193..8781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8299..8310
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8708..8728
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8795..9310)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(9416..9436)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     misc_feature    join(9447..9455,9456..9458,9459..9779,1..254)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9620..9639)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ctacagacgt tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca
       61 acagaccttg cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga
      121 cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca
      181 tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg
      241 gggagggaga ggggcggcgg ccgctcagta caagccacag ccccggagat tgttggcaat
      301 gatgatgtcg gtgacggcgt cgaataccac ctggatatta ttcgtgtctg tggcacaagt
      361 catgtgacag taaatttctt tgttgggtga gcggtttttg ctttcaaact gtgtttggat
      421 gtaggcagct gcgtcttcat aggtgttgga gcctgggtat tcaggaaagc agatggtcaa
      481 gggtgacttc ttaatcttct cgccaaagag gtctttcttg ttgaggaaga gaatgatgga
      541 ggtatcgatg aaaaacttgt tgttacagat ggagtcgaag agcatgagag actcgtgcat
      601 gcggttcgtg gtttcgtcct cgtggagcac ctggtcatag ccgctgagtg cgacacagaa
      661 gatgatggcc gtgacatcct cgaagcagtg gatccacttc ttacgttcag atcgctggcc
      721 cccaacgtca aacagcctga agtggaggtt cttgaaggtg aagtgggttt ctacgatgcc
      781 agttgttttg accctggttc ggaggatgtc ctgctcggtg ggcatgtatt ctgcagctcc
      841 aatccgatcc aagctgtcca ggtagtattt ggcagagtcg ttgagctgat actcccgaga
      901 tcggttgaag cactcctgga tccccgagtc gccccagagt cgcatcatgg cagaaagcag
      961 ttctgcagag aaagatcctc cacctccgga cttgtacagc tcgtccatgc cgagagtgat
     1021 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
     1081 gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     1141 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     1201 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
     1261 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     1321 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     1381 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     1441 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcacat
     1501 caggccgtag ccgaaggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     1561 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     1621 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     1681 gaacagctcc tcgcccttgc tcaccatgct cccgccccca ccggatcttg gttcagtgtc
     1741 ctccatgcga ctcaccacgt cacacaccat cttggagtct gccttcctct ccttgtcacc
     1801 atactccacg cccagagtat ccatggcccg cacaatggct gccagagact ggatggtgtt
     1861 gctgtagacg acaggcttgt actgctttac gtcttctcca gagaagccat cttcatggat
     1921 gatcttcatc tgcttcacaa tggtgctttt tcctgattct ccagccccca gcaggagtaa
     1981 tttcacgtct ttggcggcgc tgatgccatc ttctttgaga tttttctcaa tcgccttgct
     2041 ccgctcgagg gcggctctct cctctgcgct cagagtacat cccatggatc cgcggccgct
     2101 ctagaactag tctcgagctg cgctctcagg cacgacacga ctcctccgct gcccaccgca
     2161 gactgaggca gcgctgagtc gccggcgccg cagcgcagat ggtcgcgccc gtgcccccct
     2221 atctcgcgcc tcgcgtggtg cggtccggct gggccggcgg cggcgcggac gcgaccaagg
     2281 tggccgggaa ggggagtttg cgggggaccg gcgagtgacg tcagcgcgcc ttcagtgctg
     2341 aggcggcggt ggcgcgcgcc gccaggcggg ggcgaaggca ctgtccgcgg tgctgaagct
     2401 ggcagtgcgc acgcgcctcg ccgcatcctg tttcccctcc ccctctctga taggggatgc
     2461 gcaatttggg gaatgggggt tgggtgcttg tccagtgggt cggggtcggt cgtcaggtag
     2521 gcacccccac cccgcctcat cctggtccta aaacccactt gcactatcga taaactggat
     2581 ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt
     2641 ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc
     2701 caatcccccc ttttctttta aaggcgatac cgtcgagatc cgttcactaa tcgaatggat
     2761 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
     2821 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
     2881 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
     2941 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
     3001 tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag
     3061 gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga
     3121 ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg
     3181 caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt
     3241 tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga
     3301 tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat
     3361 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
     3421 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
     3481 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
     3541 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
     3601 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     3661 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     3721 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     3781 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     3841 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     3901 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     3961 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     4021 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     4081 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     4141 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     4201 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     4261 ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt
     4321 atatgcagga tctgagggct cgccactccc cagtcccgcc caggccacgc ctccctggaa
     4381 agtccccagc ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg aagtactccg
     4441 gatgcagctc tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc cactctaaca
     4501 cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag ctggtgttct
     4561 ctcctttatt ggcctcttct accttatctg gctcaactgg tactagcttg tagcaccatc
     4621 caaaggtcag tggatatctg acccctggcc ctggtgtgta gttctgctaa tcagggaagt
     4681 agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttctttgg gagtgaatta
     4741 gcccttccaa ctactaagtt tgtagtacat atttaacaaa tacaatttct ttaaaatgaa
     4801 aataattcag aggaatcaca ggtttagagt aaatgaaacc acaggtaatt ggcagtggta
     4861 atagggtatg gggtgggaag tttgggatga ttttggttag cttgagttat ccagttgatc
     4921 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     4981 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     5041 ataaacaagt tgatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg
     5101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5161 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5221 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5281 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5341 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5401 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5461 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5521 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5581 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5641 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5701 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5761 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5821 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5881 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5941 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6001 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6061 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6121 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6181 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6241 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6301 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6361 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6421 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6481 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6541 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6601 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6661 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6721 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6781 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6841 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6901 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6961 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7021 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7081 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7141 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7201 atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg
     7261 taaacgctag caatgtcaag gcctctcact ctctgatatt catttctttg caagttataa
     7321 atactgaata ataagatgac atgaactact actgctagag attttccaca ctgactaaaa
     7381 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     7441 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     7501 ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata
     7561 tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt
     7621 ccccagcgga aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat
     7681 gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt
     7741 ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc
     7801 ctttattggc ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa
     7861 aggtcagtgg atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc
     7921 cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc
     7981 cttccagtcc cctcttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
     8041 ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt
     8101 ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt
     8161 tttctaggtc tcgactgcag aattaattcc aggcggggag gcggcccaaa gggagatccg
     8221 actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg
     8281 ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag
     8341 aatccaggtg gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac
     8401 cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc
     8461 gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca
     8521 ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc
     8581 acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa
     8641 ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc
     8701 gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat
     8761 tttgtaatcc agaggttgat tgttccagac gcgtttacgc cagaatgcgt tcgcacagcc
     8821 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
     8881 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
     8941 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
     9001 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
     9061 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
     9121 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
     9181 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
     9241 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
     9301 tgaagaccat ggatccaccg gtgccacctc gagaaagtcg accttcttca cccacccaaa
     9361 taccatggta gtaacctcga gaagatccac caagcttgat tccggaattc gacgtcttgc
     9421 tcttgctact ccccatcttt ggtacgggtt gtggcaagct tatcatcgtg tttttcaaag
     9481 gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca
     9541 tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc
     9601 atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat
     9661 ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg
     9721 cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcg
//
