LOCUS       sequence-405606-        9576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-SV40-Galpha-oA ONE-GO
FEATURES             Location/Qualifiers
     source          1..9576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     144..161
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(1065..1775,1776..1778,1779..1781))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1107..1128)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1456..1475
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1715..1736
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(2192..2388)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(2200..2410)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2206..2341
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    2478..2593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(2856..2900)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3085..3318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3815..3940
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3987..4620
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4788..4922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4812..4831)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4866..4885
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4970..5074
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5075..5143,5144..5935)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5293..5312)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6106..6694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6595..6614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6848..6865
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6982..7003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7018..7023,7024..7041,7042..7048)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7056..7072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7061..7083
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7080..7096
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7080..7096
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7224..7857
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8064..8652
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8170..8181
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8579..8599
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8666..9181)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(9287..9307)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     misc_feature    join(9318..9326,9327..9329,9330..9576,1..328)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9491..9510)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 atcttataca cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg
       61 cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc
      121 cttcacgaca ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat
      181 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
      241 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
      301 aacgttaggg gggggggagg gagaggggcg gcggccgctc agtacaagcc acagccccgg
      361 agattgttgg caatgatgat gtcggtgacg gcgtcgaata ccacctggat attattcgtg
      421 tctgtggcac aagtcatgtg acagtaaatt tctttgttgg gtgagcggtt tttgctttca
      481 aactgtgttt ggatgtaggc agctgcgtct tcataggtgt tggagcctgg gtattcagga
      541 aagcagatgg tcaagggtga cttcttaatc ttctcgccaa agaggtcttt cttgttgagg
      601 aagagaatga tggaggtatc gatgaaaaac ttgttgttac agatggagtc gaagagcatg
      661 agagactcgt gcatgcggtt cgtggtttcg tcctcgtgga gcacctggtc atagccgctg
      721 agtgcgacac agaagatgat ggccgtgaca tcctcgaagc agtggatcca cttcttacgt
      781 tcagatcgct ggcccccaac gtcaaacagc ctgaagtgga ggttcttgaa ggtgaagtgg
      841 gtttctacga tgccagttgt tttgaccctg gttcggagga tgtcctgctc ggtgggcatg
      901 tattctgcag ctccaatccg atccaagctg tccaggtagt atttggcaga gtcgttgagc
      961 tgatactccc gagatcggtt gaagcactcc tggatccccg agtcgcccca gagtcgcatc
     1021 atggcagaaa gcagttctgc agagaaagat cctccacctc cggacttgta cagctcgtcc
     1081 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     1141 tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc gggcagcagc
     1201 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     1261 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
     1321 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
     1381 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     1441 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     1501 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     1561 cgggcgaagc acatcaggcc gtagccgaag gtggtcacga gggtgggcca gggcacgggc
     1621 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     1681 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     1741 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgctcccgcc cccaccggat
     1801 cttggttcag tgtcctccat gcgactcacc acgtcacaca ccatcttgga gtctgccttc
     1861 ctctccttgt caccatactc cacgcccaga gtatccatgg cccgcacaat ggctgccaga
     1921 gactggatgg tgttgctgta gacgacaggc ttgtactgct ttacgtcttc tccagagaag
     1981 ccatcttcat ggatgatctt catctgcttc acaatggtgc tttttcctga ttctccagcc
     2041 cccagcagga gtaatttcac gtctttggcg gcgctgatgc catcttcttt gagatttttc
     2101 tcaatcgcct tgctccgctc gagggcggct ctctcctctg cgctcagagt acatcccatg
     2161 gatccgcggc cgctctagaa ctagtctcga gtttgcaaaa gcctaggcct ccaaaaaagc
     2221 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     2281 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     2341 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     2401 ttctgcctgc tggggagcct ggggactttc cacaccatcg ataaactgga tctctgctgt
     2461 ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt
     2521 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
     2581 cttttctttt aaaggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
     2641 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     2701 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     2761 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     2821 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     2881 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     2941 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     3001 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     3061 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     3121 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     3181 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     3241 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     3301 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     3361 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     3421 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt
     3481 gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg
     3541 tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg
     3601 atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac
     3661 agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg
     3721 cccatactat atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt
     3781 cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct
     3841 tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg
     3901 ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg
     3961 ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct
     4021 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     4081 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     4141 tcagatctgg tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg
     4201 atctgagggc tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag
     4261 cggaaagtcc cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct
     4321 ctcgggccac gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct
     4381 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat
     4441 tggcctcttc taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca
     4501 gtggatatct gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg
     4561 tgtggtagat ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca
     4621 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca
     4681 gaggaatcac aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat
     4741 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
     4801 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     4861 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     4921 ttgatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4981 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5041 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5101 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5161 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5221 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5281 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5341 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5401 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5461 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5521 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5581 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5641 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5701 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5761 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5821 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5881 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5941 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6001 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6181 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6241 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6301 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6361 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6421 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6481 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6541 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6601 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6661 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6721 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6781 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6841 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6901 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6961 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7021 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7081 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtaaacgcta
     7141 gcaatgtcaa ggcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat
     7201 aataagatga catgaactac tactgctaga gattttccac actgactaaa agggtctgag
     7261 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     7321 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     7381 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     7441 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     7501 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     7561 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     7621 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     7681 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     7741 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     7801 ggtagatcca cagatcaagg atatcttgtc ttcgttggga gtgaattagc ccttccagtc
     7861 ccctcttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     7921 aaggtacctg aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct tctagccagg
     7981 cacaatcagc attggtagct gctgtattgc tacttgtgat tgctccatgt ttttctaggt
     8041 ctcgactgca gaattaattc caggcgggga ggcggcccaa agggagatcc gactcgtctg
     8101 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     8161 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     8221 ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt
     8281 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     8341 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     8401 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     8461 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     8521 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     8581 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
     8641 cagaggttga ttgttccaga cgcgtttacg ccagaatgcg ttcgcacagc cgccagccgg
     8701 tcactccgtt gatggttact cggaacagca gggagccgtc ggggttgatc aggcgctcgt
     8761 cgataatttt gttgccgttc cacagggtcc ctgttacagt gatctttttg ccgtcgaaca
     8821 cggcgatgcc ttcatacggc cgtccgaaat agtcgatcat gttcggcgta accccgtcga
     8881 ttaccagtgt gccatagtgc aggatcacct taaagtgatg atcatccaca gggtacacca
     8941 ccttaaaaat tttttcgatc tggcccattt ggtcgccgct cagaccttca tacgggatga
     9001 tgacatggat gtcgatcttc agcccatttt caccgctcag gacaatcctt tggatcggag
     9061 ttacggacac cccgagattc tgaaacaaac tggacacacc tccctgttca aggacttggt
     9121 ccaggttgta gccggctgtc tgtcgccagt ccccaacgaa atcttcgagt gtgaagacca
     9181 tggatccacc ggtgccacct cgagaaagtc gaccttcttc acccacccaa ataccatggt
     9241 agtaacctcg agaagatcca ccaagcttga ttccggaatt cgacgtcttg ctcttgctac
     9301 tccccatctt tggtacgggt tgtggcaagc ttatcatcgt gtttttcaaa ggaaaaccac
     9361 gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac atgtaaagca
     9421 tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg catccttcag
     9481 ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta tccaactcac
     9541 aacgtggcac tggggttgtg ccgcctttgc aggtgt
//
