LOCUS       sequence-405607-        9540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-SV40-Galpha-i1 ONE-GO
FEATURES             Location/Qualifiers
     source          1..9540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    178..411
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             596..640
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    903..1018
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        1086..1296
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1108..1304
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1155..1290
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1700..1702,1703..1705,1706..2416)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1745..1766)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2006..2025)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2353..2374
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3132..3706,3707..3709,3710..3718)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3299..3316)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3526..3545
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3729..3749
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3855..4370
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4384..4972
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4437..4457)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4855..4866)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5179..5812
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5940..5956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5940..5956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5953..5975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5964..5980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5988..5994,5995..6012,6013..6018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6033..6054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6171..6188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6342..6930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6422..6441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7101..7892,7893..7961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7724..7743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7962..8066)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8114..8248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8151..8170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8205..8224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8416..9049
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9096..9221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
       61 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
      121 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
      181 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
      241 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
      301 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
      361 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
      421 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
      481 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
      541 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
      601 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
      661 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
      721 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
      781 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
      841 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
      901 cctttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
      961 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     1021 gggtttatta cagggacagc agagatccag tttatcgatg gtgtggaaag tccccaggct
     1081 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     1141 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     1201 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     1261 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaactcgag actagttcta
     1321 gagcggccgc ggatccatgg gctgcacact gagcgctgag gacaaggcgg ccgtggagcg
     1381 cagcaagatg atcgaccgca acctccggga ggacggagag aaggcagcgc gcgaggtcaa
     1441 gctgctgctg ctgggtgctg gtgaatccgg gaagagcaca attgtgaagc agatgaaaat
     1501 tatccacgag gctggctact cagaggaaga gtgtaagcag tacaaagcag tggtctacag
     1561 caacaccatc cagtccatca ttgccatcat tagagccatg gggagattga aaatcgactt
     1621 tggagacgct gctcgtgcgg atgatgctcg ccaactcttc gtgcttgctg gggctgcaga
     1681 ggaaggcttt atgaccgcga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1741 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1801 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1861 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg
     1921 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1981 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2041 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2101 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2161 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2221 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2281 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     2341 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2401 ggacgagcta tacaaggcgg agctcgccgg cgtcataaag agactgtgga aggacagcgg
     2461 tgtgcaagcc tgcttcaaca gatcccggga gtaccagctg aacgattcgg cggcgtacta
     2521 cctgaatgac ttggacagaa tagcacaacc aaattacatc ccaacccagc aggatgttct
     2581 cagaactaga gtgaaaacga cgggaattgt ggaaacccac tttactttca aagatcttca
     2641 ttttaaaatg tttgacgtgg gaggccagag atcagagcgg aagaagtgga ttcactgctt
     2701 tgaaggcgtg actgccatca tcttctgtgt ggccctgagt gactatgacc tggttcttgc
     2761 tgaggatgaa gaaatgaacc ggatgcacga aagcatgaag ctgttcgata gcatatgtaa
     2821 caacaagtgg tttacggaca catccatcat ccttttcctg aacaagaagg acctcttcga
     2881 agagaagatc aaaaagagtc ccctcacgat atgctatcca gaatatgcag gctcaaacac
     2941 atatgaagag gcggctgcgt atatccagtg tcagtttgaa gacctcaata aaaggaagga
     3001 cacaaaggaa atttacaccc acttcacttg cgccacggat acgaagaatg tgcagtttgt
     3061 gttcgatgct gtaacggacg tcatcataaa gaataaccta aaagactgtg gtctcttcta
     3121 agcggccgcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     3181 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     3241 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     3301 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     3361 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     3421 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     3481 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     3541 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     3601 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     3661 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg
     3721 taccaaagat ggggagtagc aagagcaaga cgtcgaattc cggaatcaag cttggtggat
     3781 cttctcgagg ttactaccat ggtatttggg tgggtgaaga aggtcgactt tctcgaggtg
     3841 gcaccggtgg atccatggtc ttcacactcg aagatttcgt tggggactgg cgacagacag
     3901 ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc
     3961 tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga
     4021 tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg
     4081 aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact
     4141 atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt
     4201 atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg
     4261 gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa
     4321 ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa acgcgtctgg
     4381 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4441 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4501 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4561 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4621 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4681 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4741 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4801 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4861 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4921 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     4981 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     5041 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     5101 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5161 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     5221 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     5281 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     5341 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     5401 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     5461 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     5521 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     5581 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     5641 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5701 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5761 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     5821 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     5881 aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg
     5941 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6001 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6061 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6121 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6181 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6241 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7081 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7201 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7261 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7381 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7621 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7741 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7801 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7861 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7921 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7981 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8041 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     8101 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8161 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8221 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     8281 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     8341 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     8401 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     8461 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     8521 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     8581 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     8641 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     8701 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     8761 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     8821 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     8881 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8941 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9001 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     9061 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     9121 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     9181 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     9241 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     9301 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     9361 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     9421 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     9481 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
//
