LOCUS       sequence-405618-        2783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE
            overhangs used to build a level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_DE_mTurquoise2
FEATURES             Location/Qualifiers
     source          1..2783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             415..1206
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSAYPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1297..1885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1786..1805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2031..2744,2745..2747,2748..2750))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2076..2097)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2425..2444
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2684..2705
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agaatggatg cgagtaatga gctcggcaca aaatcaccac tcgatacagg cagcccatca
       61 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
      121 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
      181 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac cgctcctcta
      241 gagtcgactt gcaggcatgc aagcttagca agcgaaccgg aattgccagc tggggcgccc
      301 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
      361 tgatggcgca ggggatcaat atctgatcaa gagacaggat gaggatcgtt tcgcatgagg
      421 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
      481 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
      541 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
      601 cggcgagctt tgatcaacga ccttttggaa acttcggctt accctggaga gagtgagatt
      661 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
      721 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc tggtatcttc
      781 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
      841 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
      901 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
      961 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     1021 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     1081 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     1141 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     1201 aaataataat cacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtgggtgg
     1981 aagggctcgg agttgtggta atctatgtat cctggtggtc tctaagctta tcacttgtac
     2041 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     2101 tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg
     2161 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     2221 acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc
     2281 ttgtcggcgg tgatatagac gttgtcgcta aagtagttgt actccagctt gtgccccagg
     2341 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     2401 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     2461 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     2521 tcggggtagc gggcgaagca ctgcacgccc caggacaggg tggtcacgag ggtgggccag
     2581 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     2641 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     2701 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat accttgagac
     2761 ctgagacgag acgagacagc ctg
//
