LOCUS       sequence-405624-        2549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 47740 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-47740
FEATURES             Location/Qualifiers
     source          1..2549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             414..1205
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1296..1884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1785..1804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaatggatgc gagtaatgag ctcggcacaa aatcaccact cgatacaggc agcccatcag
       61 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
      121 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
      181 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gctcctctag
      241 agtcgacttg caggcatgca agcttagcaa gcgaaccgga attgccagct ggggcgccct
      301 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
      361 gatggcgcag gggatcaata tctgatcaag agacaggatg aggatcgttt cgcatgaggg
      421 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      481 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      541 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
      601 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agtgagattc
      661 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
      721 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgct ggtatcttcg
      781 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
      841 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
      901 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
      961 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1021 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1081 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     1141 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     1201 aataataatc acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga
     1261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1381 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1441 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1501 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1561 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1621 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1681 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtgggtgga
     1981 agggctcgga gttgtggtaa tctatgtatc ctggtggtct caagcgacat tgatcctcga
     2041 aggtgatgcc gaagctacca gagatgaaac atggtccggc caaaagttgc gtttggcggg
     2101 cacgctgcca gcatcgtccg atcccgtgta cgtcttggtg acaatcacgg ggtctggtat
     2161 tacgggcccg ggtagtgccg gtggtggatg ccaggcactc ttgtctgttt gttccgacaa
     2221 cgcattggcc gtgaatagca ttttgaacac gttgaaaaag acggttcaga acttgagcga
     2281 tacggaaaca cagtagtctt ctatagccag ataggtagta cacaaaaaaa aactctgtct
     2341 agagcaactc ttcgcgaaac cgtaaacgtc ttaagataac gcagccgtaa aggtacaaaa
     2401 gtagctaact acagcaatga ggagtgagga gagtacaaat catctacaag ttcattcaaa
     2461 tgtaaacgtt aattcgtatt aggtaggcgt gggctgcgcg gatcgtttgg gatataagct
     2521 gagacctgag acgagacgag acagcctga
//
