LOCUS       sequence-405626-        2287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 51183 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-51183
FEATURES             Location/Qualifiers
     source          1..2287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             387..1178
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1269..1857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1758..1777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
       61 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
      121 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
      181 gagcgttgct gcctgtgatc accgctcctc tagagtcgac ttgcaggcat gcaagcttag
      241 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
      301 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca atatctgatc
      361 aagagacagg atgaggatcg tttcgcatga gggaagcggt gatcgccgaa gtatcgactc
      421 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
      481 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
      541 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
      601 aaacttcggc ttcccctgga gagagtgaga ttctccgcgc tgtagaagtc accattgttg
      661 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
      721 ggcagcgcaa tgacattctt gctggtatct tcgagccagc cacgatcgac attgatctgg
      781 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
      841 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
      901 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
      961 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     1021 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     1081 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     1141 acgtgaaagg cgagatcacc aaggtagtcg gcaaataata atcacctaga atgcatgacc
     1201 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1261 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1321 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1381 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1441 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1501 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1561 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1621 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1681 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1741 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1801 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1861 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1921 tttcctgcgt tatcccctga ttctgtgggt ggaagggctc ggagttgtgg taatctatgt
     1981 atcctggtgg tctcaagcgg gatcaatctt atgagtgtct ccaatatgct tcatgaggaa
     2041 aaaaaatcct atgaatcaat agtttgtctc tatgctaatt acgatccgag caatggagag
     2101 cataaatggc aaatctatga ggactactta caattactgt cagagcatat tttttacatc
     2161 aaatttgtaa ccttaagata tatgctgcct actgagcttc ggaaatttga aaaggcaact
     2221 ttccctaagc tgagacctga gacgagacga gacagcctga gaatggatgc gagtaatgag
     2281 ctcggca
//
