LOCUS       sequence-405636-       17159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An. gambiae transgenesis plasmid. Expresses gRNAs targeting 
            B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17159)
  AUTHORS   Smidler AL, Apte RA, Pai JJ, Chow ML, Chen S, Mondal A, Sanchez C 
            HM, Antoshechkin I, Marshall JM, Akbari OS
  TITLE     Eliminating Malaria Vectors with Precision Guided Sterile Males.
  JOURNAL   bioRxiv [Preprint]. 2023 Jul 21:2023.07.20.549947. doi: 
            10.1101/2023.07.20.549947.
  PUBMED    37503146
REFERENCE   2  (bases 1 to 17159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Jul 21:2023.07.20.549947. doi: 
            10.1101/2023.07.20.549947."
COMMENT     Sequence Label: 1114H
FEATURES             Location/Qualifiers
     source          1..17159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    993..1026
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        1400..1475
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    2514..2635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(2758..3471,3472..3474,3475..3477))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_RNA        complement(5688..5763)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(6163..6238)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(9282..9284,9285..9287,9288..9998)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    10128..10209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10235..10268
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     10272..10288
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     10273..10289
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   10572..10606
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11097..11113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11097..11113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11110..11132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11121..11137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11145..11151,11152..11169,11170..11175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11190..11211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11328..11345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15481..16069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15561..15580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(16287..16306)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             16298..17107
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(16365..16384)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcgaattc atataacttc gtataatgta tgctatacga
     1021 agttatggat cggcctagca agatcaggtc tcaatcccca catatacact gaagcggttt
     1081 ggctcttggt tattctagaa aactctgttt ttgaattaat cataggaaaa ctgtttattc
     1141 ttttttgata tttcaacgta ttacaatgaa tagtaacttt tgattaaaaa agagatgaag
     1201 caatacaaga aatgttgaaa agtttatgaa acatcacatt agcgtgagtt acggcaggat
     1261 caaaacctta taacagtcac actcaggcaa aaaatccttc ttgaatatcc tttatgcatc
     1321 gctagagcaa ggattgaaag cgcaaagtat atatacaacc ttttttcccc tcgtccttgg
     1381 atccgatcac gcagtcgatg ttttagagct agaaatagca agttaaaata aggctagtcc
     1441 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tccaccactc ttcacgccta
     1501 aagcataaac gtcgagcagt catgaaagtc tccacatata cactgaagcg gtttggctct
     1561 tggttattct agaaaactct gtttttgaat taatcatagg aaaactgttt attctttttt
     1621 gatatttcaa cgtattacaa tgaatagtaa cttttgatta aaaaagagat gaagcaatac
     1681 aagaaatgtt gaaaagttta tgaaacatca cattagcgtg agttacggca ggatcaaaac
     1741 cttataacag tcacactcag gcaaaaaatc cttcttgaat atcctttatg catcgctaga
     1801 gcaaggattg aaagcgcaaa gtatatatac aacctttttt cccctcgtcc ttggctcgat
     1861 atcgtgcgca agggttccag agctatggaa acatagcaag ttggaataag gcttagtccg
     1921 tactcaactt gaaaaagtgg caccgagtcg gtgcattttt tggcttgaga cctgatctgg
     1981 cgcgcctaag gtgctttaag aaccggacga cggtctcact agcagtcatg aaagtctcca
     2041 catatacact gaagcggttt ggctcttggt tattctagaa aactctgttt ttgaattaat
     2101 cataggaaaa ctgtttattc ttttttgata tttcaacgta ttacaatgaa tagtaacttt
     2161 tgattaaaaa agagatgaag caatacaaga aatgttgaaa agtttatgaa acatcacatt
     2221 agcgtgagtt acggcaggat caaaacctta taacagtcac actcaggcaa aaaatccttc
     2281 ttgaatatcc tttatgcatc gctagagcaa ggattgaaag cgcaaagtat atatacaacc
     2341 ttttttcccc tcgtccttgc caaataggcg ctaagttctg ttccagagct atggaaacat
     2401 agcaagttgg aataaggctt agtccgtact caacttgaaa aagtggcacc gagtcggtgc
     2461 attttttcta gtgagacctg accacagagc ataaggagaa cctcgcctag gtttaagata
     2521 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2581 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2641 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2701 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc ggccgcttta
     2761 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     2821 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg
     2881 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     2941 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     3001 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     3061 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     3121 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     3181 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     3241 catgtggtcg gggtagcggg cgaagcactg caggccgtag ccgaaggtgg tcacgagggt
     3301 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     3361 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     3421 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatatt
     3481 gtttcctttc tttattcacc ggaagatgac aattctcctt tgggaatgta cgattcgaga
     3541 tctgtgtgtc tcccgaagat gaaaaaagct gttggtttca accaaaaaac gtgtccgttg
     3601 ctcgaagcgc gactaacggc gactctcttt cgactagcaa tggcgtccgc aggcacaaac
     3661 cgagaggaat agtggaacat gcaatgcagc agcagcagca cgtggagaga gagaaagaaa
     3721 gatggtcgaa atattataaa aaataattgc cgttgcacac acacacacta caccaccact
     3781 atcgacgacc accgctctac ccgcactcat agcaacctaa agcgacgatg gtattttcca
     3841 gcgaaatttt cattgtccat tgcaagcgac ctcacacacc gctaccgaag tagcctttga
     3901 agcacgcgcg cgtgtgaagg cgtgtgcctc tgtgtgtgta ggtgaatatc actgtgtaca
     3961 ctgcgctaag ccagtggcgc gcgcgctttt cgcatcaatt attgccggat gaacgacaac
     4021 gagatgctct tcgcggggcc cgctgtcccg gaacgtgcac gaaagcacca tttctcctct
     4081 cggaaactcc acatctcgga aagcttccca actatgcccc aacgtctctc ttatctattg
     4141 catatcgaag acttatgaat ccgctccagt gccaactgcg gttcatgaaa cacaaaacgg
     4201 attttcaccc ctgattcgag cacacaagag caccatggta agctgtggaa ataacgccat
     4261 ccttctggat atcttgtaac gaaaaaggcc aaggactgtg attctactaa cctcactcag
     4321 gaactgatat gagagcacac agagccacac aaccactcca cgacgggata cgcttggatt
     4381 aaaactaaat cgcttccgta cgtcgacacc gactggcccc tataccgctt ctcaaataac
     4441 gacgatctca gctaaaatgc cccccacaca catgcatgcg cagcacactt gttcacttcc
     4501 acttccttgc ctgctctgtg tgcggtggtg gatacgtgct gtgctgtaag aacacttcac
     4561 acagaaagga agggacagag caaaccaaca cactgccacc gctcttctcg tgccatttcc
     4621 ttagaaaatt aatccaaaaa ctcaactgcg ccacacacca cggcaacctt ttgctatcat
     4681 aaggggggag gcgcgcaata tgcacaacac acgatacaca ccacggaatg tttacgaaca
     4741 gcgtgcgaac aggcaatttc ggtaccggcg gcaacagccg attttgacag agcgctcaac
     4801 acacacacgc acacactcta ataccagcaa cgcgtgcgaa ggcttttgtt tcgcactgcc
     4861 agtgtgttag tgggagcagg tacaaaatgt aacaaagggc gaatggcgcg tgctactgaa
     4921 caatacaagc aaattcgaga aaaattaaat aagccaagag attattttat gagtaaatga
     4981 aatgaaattt aatttaactg tattttgaac ataactaaga aaatactaat caactggatg
     5041 aaataatata acatcacaaa acaataattc aatttgaaat cagttacgtt agttttgccg
     5101 aaatgagaga tttttttcac aaacacttca ctaacttcta acaaacaaac atttgttcta
     5161 taaaactagt tcacttacct gagttttgaa gtactattcg gctggttatt tcacttgcaa
     5221 aagcaattgg ctaatttagc gctaagcacg tcagcagaaa cctccgactc gccgagagta
     5281 ctccgagcaa aagacagcaa aatgctggat tttgcaccca cacggcctac tcgtccgtcg
     5341 tactgtgcaa cttggatgga agatttcgac acgggactct acagggaggg cttatatgtg
     5401 ttttataatg ctgtatgtat gtttttatgt ataaaaaatg caaaaagtat gtctttttag
     5461 tttattataa caaataaaat aaaatacaaa ttataaaaca atcaatttct caacggtttt
     5521 gtttgcagcg agttgtgatt tgcttgaaaa caatgcgcac gcggtaattt tcccgcccag
     5581 ctcggcatgt caaacacgtc tgacgttagg cttcccactg tggctgctgc tgtcatggaa
     5641 ggaaaagaat ttgctcgcgt tctacatcgc tagctcaatc gaaaaaagca ccgactcagt
     5701 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
     5761 aaccccgtca gagtggatga taacaaggac gaggggaaaa aaggttgtat atatactttg
     5821 cgctttcaat ccttgctcta gcgatgcata aaggatattc aagaaggatt ttttgcctga
     5881 gtgtgactgt tataaggttt tgatcctgcc gtaactcacg ctaatgtgat gtttcataaa
     5941 cttttcaaca tttcttgtat tgcttcatct cttttttaat caaaagttac tattcattgt
     6001 aatacgttga aatatcaaaa aagaataaac agttttccta tgattaattc aaaaacagag
     6061 ttttctagaa taaccaagag ccaaaccgct tcagtgtata tgtgggctat aatttgcctt
     6121 gcatataagt tgcgttactt cagcgtccta accgcaaaaa atgcaccgac tcggtgccac
     6181 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaaccc
     6241 cgtcagagtg gatgataaca aggacgaggg gaaaaaaggt tgtatatata ctttgcgctt
     6301 tcaatccttg ctctagcgat gcataaagga tattcaagaa ggattttttg cctgagtgtg
     6361 actgttataa ggttttgatc ctgccgtaac tcacgctaat gtgatgtttc ataaactttt
     6421 caacatttct tgtattgctt catctctttt ttaatcaaaa gttactattc attgtaatac
     6481 gttgaaatat caaaaaagaa taaacagttt tcctatgatt aattcaaaaa cagagttttc
     6541 tagaataacc aagagccaaa ccgcttcagt gtatatgtgg gctttgagac ctgatcttgc
     6601 taccggtcgc atgtgcttgt gtgtgaggcg tctctctctt cgtctctgtt gcgcaaacgc
     6661 atagactgca ctgaaaaaat cgattaccta ttttttatga atgaatattt gcactattac
     6721 tattcaaaac tattaagata gcaatcacat tcaatagcca aatactatac cacctgagcg
     6781 atgcaacgaa atgatcaatt tgagcaaaaa tgctgcatat ttaggacggc atcattatag
     6841 aaatgcttct tgctgtgtac ttttctctcg tctggcagct gtttcgccgt tattgttaaa
     6901 accggcttaa gttaggtgtg ttttctacga ctagtgaatg ccctactaga agatgtgtgt
     6961 tgcacaaaat gtccctggaa taaccaattt gaagtgcaga tagcagtaaa cgtaagctaa
     7021 tatgaatatt atttaactgt aatgttttaa tatcgctgga cattactaat aaacccacta
     7081 taaacacatg tacatatgta tgttttggca tacaatgagt agttggggaa aaaatgtgta
     7141 aaagcaccgt gaccatcaca gcataaagat aaccagctga agtatcgaat atgagtaacc
     7201 cccaaattga atcacatgcc gcaactgata ggacccatgg aagtacactc ttcatggcga
     7261 tatacaagac acacacaagc acgaacaccc agttgcggag gaaattctcc gtaaatgaaa
     7321 acccaatcgg cgaacaattc atacccatat atggtaaaag ttttgaacgc gacttgagag
     7381 cggagagcat tgcggctgat aaggttttag cgctaagcgg gctttataaa acgggctgcg
     7441 ggaccagttt tcatatcact accgtttgag ttcttgtgct gtgtggatac tcctcccgac
     7501 acaaagccgc tccatcagcc agcagtcgtc taatccagag acaccaaacc gaaagactta
     7561 atttatattt atttaattaa ttttaataaa acacaccaaa tgtaagtagc tttccccttc
     7621 ccaacaacaa aacaccatcg aaccactccc accaagaaaa agcaataatc gagaaaagcc
     7681 gcggaaaatg tgtgattttt tttgtaaaca aaacattttt ttatgtgcca gtgctgaaag
     7741 tgatcaaaaa atactagcca cgagctaaag agttattgta ttgaccaaaa ctccaaaaat
     7801 acccaagttt ggccctaaat tgtcaatcaa aataccaata ggtcgaaaga catcaaaatt
     7861 aacaaaacca gggtttcaaa taccataact caagaatcag gattacaact gcagatttca
     7921 ggatatatac atacaaatta tacgaaatta taaaaaccaa agcaattcaa tagccccaac
     7981 tcaaatgtta ggatctaata tagtgtttaa agccaagctc gctgatgtgg gcgtgtcacg
     8041 atttcaccca aagatatgcc aaattacgaa ttgcaaatca attcgccaac actttctttt
     8101 tttcccacgc cctaaaacac cagatcatcc ataaatgtac atacatacag tatatgcata
     8161 ttataatcta taaaactaga tcaggttctt gaaaatagtg acgtaggcag ccgttttggc
     8221 ttaagcagaa atttttgccg gtttttcaaa gttgtagttg caaaaatgga gaaaaccttc
     8281 gagcattcgt tcatgtacac acactcacgc gcaaaataac gagagagagt gtatgtgtgt
     8341 gtgagagagc gaaagccaga cgacggtttg cttttcgcct cgaaacatga ccatatatgg
     8401 tcacaaaact tggccgccgc aattcaacac accagcgctc tccttcgcac ccatagcgac
     8461 catggcgcgg agcgagcgag atggcgagag cgagcgacgc ctatggcgac gtcgacgcag
     8521 gcagcgattg aaaaacgcag ttaactggca ttcaacattc accagccact ttcagtcggt
     8581 ttattccagt cattcctttc aaaccgtgcg gtcgcttagc tcagcctcgc cacttgcgtt
     8641 tacagtagtt ttcacgcctt gaatttgtta aatcgaacaa aaaggtaaag tttaactagc
     8701 tttgaaaagt ttcgtggctc ttaattgtta aattttctag agtgcgttta gtgttttttt
     8761 ttttattttg taatgttaat ttcgggttcc aattcgagtt ttaggcagcc gccattttaa
     8821 gggcgcatac acacaggcaa ctgtgctctc tttgcggctt tcttttgcac cggcattcgt
     8881 taagtgtcgt ctagaagctt ctcccctccc ttttcggcat attcgtattg tggttttaat
     8941 ttttcggggc ggggccttct attttgtaac tgttctttta atttcttatt acaattcgat
     9001 cgcaagtgaa aatcagtttt caatcggaaa agtatttttt tatgaaattt ttttttgtcc
     9061 aagattaaaa ttttgtacta aaaaaacgta cattgcattg agtgattttt aattgtacac
     9121 gaaaaacaag ttagtttgtt atgacaattg tattttggta aatcagcgca gtccaaggaa
     9181 accacgcaaa ttctcagttt ttttttttgc catttctaca ttaccaaata aggtaaccaa
     9241 aaactaatgg gaaatccgca ttctttccat tgcagcttac aatggtgagc aagggcgagg
     9301 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     9361 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     9421 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct
     9481 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     9541 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     9601 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     9661 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact
     9721 ttagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     9781 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     9841 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
     9901 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     9961 ccgccgggat cactctcggc atggacgagc tgtacaagtc cggatgacgg ccgcgactct
    10021 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
    10081 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    10141 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    10201 tttcactgca ttctagttgt ggtttgtcca aactataact tcgtataatg tatgctatac
    10261 gaagttatta ctcgaggtcg acggtatcga taagcttgat atctataaca agaaaatata
    10321 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
    10381 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
    10441 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
    10501 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
    10561 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa tctagctgca
    10621 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
    10681 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
    10741 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
    10801 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
    10861 ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
    10921 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
    10981 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
    11041 gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta atcatggtca
    11101 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    11161 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    11221 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    11281 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    11341 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    11401 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgcgatgtag aacgcgagca
    11461 aattcttttc cttccatgac agcagcagcc acagtgggaa gcctaacgtc agacgtgttt
    11521 gacatgccga gctgggcggg aaaattaccg cgtgcgcatt gttttcaagc aaatcacaac
    11581 tcgctgcaaa caaaaccgtt gagaaattga ttgttttata atttgtattt tattttattt
    11641 gttataataa actaaaaaga catacttttt gcatttttta tacataaaaa catacataca
    11701 gcattataaa acacatataa gccctccctg tagagtcccg tgtcgaaatc ttccatccaa
    11761 gttgcacagt acgacggacg agtaggccgt gtgggtgcaa aatccagcat tttgctgtct
    11821 tttgctcgga gtactctcgg cgagtcggag gtttctgctg acgtgcttag cgctaaatta
    11881 gccaattgct tttgcaagtg aaataaccag ccgaatagta cttcaaaact caggtaagtg
    11941 aactagtttt atagaacaaa tgtttgtttg ttagaagtta gtgaagtgtt tgtgaaaaaa
    12001 atctctcatt tcggcaaaac taacgtaact gatttcaaat tgaattattg ttttgtgatg
    12061 ttatattatt tcatccagtt gattagtatt ttcttagtta tgttcaaaat acagttaaat
    12121 taaatttcat ttcatttact cataaaataa tctcttggct tatttaattt ttctcgaatt
    12181 tgcttgtatt gttcagtagc acgcgccatt cgccctttgt tacattttgt acctgctccc
    12241 actaacacac tggcagtgcg aaacaaaagc cttcgcacgc gttgctggta ttagagtgtg
    12301 tgcgtgtgtg tgttgagcgc tctgtcaaaa tcggctgttg ccgccggtac cgaaattgcc
    12361 tgttcgcacg ctgttcgtaa acattccgtg gtgtgtatcg tgtgttgtgc atattgcgcg
    12421 cctccccccc ttatgatagc aaaaggttgc cgtggtgtgt ggcgcagttg agtttttgga
    12481 ttaattttct aaggaaatgg cacgagaaga gcggtggcag tgtgttggtt tgctctgtcc
    12541 cttcctttct gtgtgaagtg ttcttacagc acagcacgta tccaccaccg cacacagagc
    12601 aggcaaggaa gtggaagtga acaagtgtgc tgcgcatgca tgtgtgtggg gggcatttta
    12661 gctgagatcg tcgttatttg agaagcggta taggggccag tcggtgtcga cgtacggaag
    12721 cgatttagtt ttaatccaag cgtatcccgt cgtggagtgg ttgtgtggct ctgtgtgctc
    12781 tcatatcagt tcctgagtga ggttagtaga atcacagtcc ttggcctttt tcgttacaag
    12841 atatccagaa ggatggcgtt atttccacag cttaccatgg tgctcttgtg tgctcgaatc
    12901 aggggtgaaa atccgttttg tgtttcatga accgcagttg gcactggagc ggattcataa
    12961 gtcttcgata tgcaatagat aagagagacg ttggggcata gttgggaagc tttccgagat
    13021 gtggagtttc cgagaggaga aatggtgctt tcgtgcacgt tccgggacag cgggccccgc
    13081 gaagagcatc tcgttgtcgt tcatccggca ataattgatg cgaaaagcgc gcgcgccact
    13141 ggcttagcgc agtgtacaca gtgatattca cctacacaca cagaggcaca cgccttcaca
    13201 cgcgcgcgtg cttcaaaggc tacttcggta gcggtgtgtg aggtcgcttg caatggacaa
    13261 tgaaaatttc gctggaaaat accatcgtcg ctttaggttg ctatgagtgc gggtagagcg
    13321 gtggtcgtcg atagtggtgg tgtagtgtgt gtgtgtgcaa cggcaattat tttttataat
    13381 atttcgacca tctttctttc tctctctcca cgtgctgctg ctgctgcatt gcatgttcca
    13441 ctattcctct cggtttgtgc ctgcggacgc cattgctagt cgaaagagag tcgccgttag
    13501 tcgcgcttcg agcaacggac acgttttttg gttgaaacca acagcttttt tcatcttcgg
    13561 gagacacaca gatctcgaat cgtacattcc caaaggagaa ttgtcatctt ccggtgaata
    13621 aagaaaggaa acaatatggg ctctagcctg gacgacgagc acatcctgag cgccctgctg
    13681 cagagcgacg acgaactggt gggcgaggac agcgacagcg aggtcagcga ccacgtgtcc
    13741 gaggacgacg tgcagtccga caccgaggaa gccttcatcg acgaggtgca cgaagtgcag
    13801 cctaccagca gcggctccga gatcctggac gagcagaacg tgatcgagca gcctggcagc
    13861 tccctggcca gcaacagaat cctgaccctg ccccagagaa ccatcagagg caagaacaag
    13921 cactgctggt ccacctccaa gagcaccagg cggagcagag tgtccgccct gaacatcgtg
    13981 cggagccaga ggggccccac cagaatgtgc agaaacatct acgaccccct gctgtgcttc
    14041 aagctgttct tcaccgacga gatcatcagc gagatcgtga agtggaccaa cgccgagatc
    14101 agcctgaaga ggcgggagag catgaccagc gccaccttca gagacaccaa cgaggacgag
    14161 atctacgcct tcttcggcat cctggtgatg accgccgtga gaaaggacaa ccacatgagc
    14221 accgacgacc tgttcgacag atccctgagc atggtgtacg tgtccgtgat gagcagagac
    14281 agattcgact tcctgatcag atgcctgaga atggacgaca agagcatcag acccaccctg
    14341 cgggagaacg acgtgttcac ccccgtgcgg aagatctggg acctgttcat ccaccagtgc
    14401 atccagaact acacccctgg cgcccacctg accatcgatg agcagctgct gggcttcaga
    14461 ggcagatgcc ccttcagagt gtacatcccc aacaagccca gcaagtacgg catcaagatc
    14521 ctgatgatgt gcgacagcgg caccaagtac atgatcaacg gcatgcccta cctgggcaga
    14581 ggcacccaga caaacggcgt gcccctgggc gagtactacg tgaaagaact gagcaagcct
    14641 gtgcatggca gctgcaggaa catcacctgc gacaactggt tcaccagcat ccccctggcc
    14701 aagaacctgc tgcaggaacc ctacaagctg accatcgtgg gcaccgtgcg gagcaacaag
    14761 cgggagatcc cagaggtgct gaagaacagc agatccagac ctgtgggaac aagcatgttc
    14821 tgcttcgacg gccccctgac cctggtgtcc tacaagccca agcccgccaa gatggtgtac
    14881 ctgctgtcca gctgcgacga ggacgccagc atcaacgaga gcaccggcaa gccccagatg
    14941 gtgatgtact acaaccagac caagggcggc gtggacaccc tggaccagat gtgcagcgtg
    15001 atgacctgca gcagaaagac caacagatgg cccatggccc tgctgtacgg catgatcaat
    15061 atcgcctgca tcaacagctt catcatctac agccacaacg tgtccagcaa gggcgagaag
    15121 gtgcagagcc ggaagaaatt catgcggaac ctgtacatga gcctgacctc cagcttcatg
    15181 agaaagagac tggaagcccc caccctgaag agatacctgc gggacaacat cagcaacatc
    15241 ctgcccaagg aagtgccagg aacaagcgac gacagcaccg aggaacccgt gatgaagaag
    15301 aggacctact gcacctactg tcccagcaag atcagaagaa aggccaacgc cagctgcaag
    15361 aaatgcaaaa aagtgatctg ccgggagcac aacatcgaca tgtgccagag ctgtttctga
    15421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    15481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    15541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    15601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    15661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    15721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    15781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    15841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    15901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    15961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    16021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    16081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    16141 catgagatta tcaaaaagga tcttcaccta gatccctgtt tcctggcagc tctggcccgt
    16201 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
    16261 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
    16321 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
    16381 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
    16441 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
    16501 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
    16561 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
    16621 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
    16681 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
    16741 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
    16801 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
    16861 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
    16921 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
    16981 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
    17041 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
    17101 tttctaatca gaattggtta attggttgta acactggcca tttccccgaa aagtgccac
//
