LOCUS       sequence-405669-        2981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knockdown of FYN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Comba A, Dunn PJ, Argento AE, Kadiyala P, Ventosa M, Patel P, Zamler
            DB, Nunez FJ, Zhao L, Castro MG, Lowenstein PR
  TITLE     Fyn tyrosine kinase, a downstream target of receptor tyrosine 
            kinases, modulates antiglioma immune responses.
  JOURNAL   Neuro Oncol. 2020 Jun 9;22(6):806-818. doi: 10.1093/neuonc/noaa006.
  PUBMED    31950181
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Neuro Oncol"; date: "2020-06-9- 9"; volume: "22"; issue: "6"; 
            pages: "806-818"
COMMENT     Sequence Label: pT2/shFYN1/GFP4_Seq_B
FEATURES             Location/Qualifiers
     source          1..2981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     285..302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    418..439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(454..459,460..477,478..484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    492..508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     497..519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     516..532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     516..532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     ncRNA           complement(639..653)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(717..736)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(718..736)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(747..764)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(747..763)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(756..778)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(992..1011)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1204..1225
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1388..1492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1493..1561,1562..2353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1711..1730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2524..2981,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat ggtctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      361 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      421 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      481 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      541 cgccaagcgc gtcaattaac cctcactaaa gggaacaaaa gctgttaatt aagaattctc
      601 cgaggcagta ggcatcgatg ttatgtcaaa ggccaataca tctgtggctt cactattggc
      661 ctttgacata acatcggcgc tcactgtcaa cagcactcga gggcgcgccc aattcgccct
      721 atagtgagtc gtattacgtc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
      781 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      841 aatagcgaag aggcccgcac cgaaacgccc ttcccaacag ttgcgcagcc tgaatggcga
      901 atgggagcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      961 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     1021 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1081 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttggcctgta
     1141 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1201 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1261 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1321 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2641 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2941 agggagaaag gcggacaggt atccggtaag cggcagggtc g
//
