LOCUS       sequence-405674-        2350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 27877 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-27877
FEATURES             Location/Qualifiers
     source          1..2350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      270..858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     759..778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1738..2350,1..179)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
       61 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
      121 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
      181 aatcacctag aatgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      241 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      301 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      361 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      421 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      481 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      541 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      601 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      661 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      721 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      781 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      841 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      901 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtggg tggaagggct
      961 cggagttgtg gtaatctatg tatcctggtg gtctcgagcg ctcaaacaca actcgccaac
     1021 tgtgcaaata gacacaaacc tacttctaat gagagagctg cagtccccta tgataaataa
     1081 gaatggcaaa aggtatcgac aaactggact ttaaatactg tttaggggga gaaatagaca
     1141 tatgatcaaa aacctggaaa aacaaactaa aactgtccca tagtctatcc caaaatctca
     1201 aaatgaaaaa ttcaaagcca ctacaccaaa gaaacgatat aaacatgaat cagacgggct
     1261 gaaagtccac acgagcagca attaaaccgt aagctgagac ctgagacgag acgagacagc
     1321 ctgagaatgg atgcgagtaa tgagctcggc acaaaatcac cactcgatac aggcagccca
     1381 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     1441 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     1501 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caccgctcct
     1561 ctagagtcga cttgcaggca tgcaagctta gcaagcgaac cggaattgcc agctggggcg
     1621 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     1681 atctgatggc gcaggggatc aatatctgat caagagacag gatgaggatc gtttcgcatg
     1741 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     1801 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     1861 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     1921 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagtgag
     1981 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2041 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgctggtatc
     2101 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2161 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2221 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2281 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2341 atcgcgccga
//
