LOCUS       sequence-405675-        2278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 18049 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-fcpA
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             408..1199
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1290..1878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1779..1798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcgagtaa tgagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
       61 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
      121 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
      181 atgttgattg taacgatgac agagcgttgc tgcctgtgat caccgctcct ctagagtcga
      241 cttgcaggca tgcaagctta gcaagcgaac cggaattgcc agctggggcg ccctctggta
      301 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
      361 gcaggggatc aatatctgat caagagacag gatgaggatc gtttcgcatg agggaagcgg
      421 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
      481 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
      541 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
      601 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagtgag attctccgcg
      661 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
      721 gcgaactgca atttggagaa tggcagcgca atgacattct tgctggtatc ttcgagccag
      781 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
      841 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
      901 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
      961 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     1021 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     1081 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     1141 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     1201 aatcacctag aatgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1261 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1321 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1381 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1441 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1501 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1561 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1621 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1681 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1741 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1801 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1861 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1921 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtggg tggaagggct
     1981 cggagttgtg gtaatctatg tatcctggtg gtctcgagcg ttgaagacga gctagtgtta
     2041 ttcctgactg tgaaaccaaa gcggagtgac tgcaactaat gaaaattaaa tttttaagga
     2101 aggatagaga ctcccaactg ttcgtgcacc atgagagcaa ggatgcccat ttgtgccgca
     2161 cgtccctggt tgagttcgat agcacgcttc tgaagcttct tgtcctcact gaaagtgtcc
     2221 cagccaaagt cgaggtagaa gctgagacct gagacgagac gagacagcct gagaatgg
//
