LOCUS       sequence-405680-        2562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 48707 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-sit4
FEATURES             Location/Qualifiers
     source          1..2562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(851..1439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(931..950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1530..2321)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
       61 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
      121 ctgcctgtat cgagtggtga ttttgtgccg agctcattac tcgcatccat tctcaggctg
      181 tctcgtctcg tctcaggtct cagcttattt atagttattg aacaaagagt aattctagta
      241 agtccttcat tgtcactcca gcgcaccttg cgacaaagtt tttggtgtcg gaaaagtcat
      301 ctgcgaaata cggtggatcc gcatagacaa acacgtgatt aacgtgtgtg aacaaagagg
      361 tacttctgct caggggagat tctgggcata catatataaa ttaccaaatg tagggaagcg
      421 tcagtgacgc ttgcgtctgg aaccaacaca atgcgagatc aatttgcaac attggaagca
      481 cttctctcat tgactccgct gtcccacgct aaaagggcag ccaacacaga actgtccaca
      541 cataggtgcg cacaataaac ggaaaggact ccatttagaa tgtcacgaac agtggttaag
      601 aaacgctatt ttgtacccaa atgtgtggcg atatagttca cgtaaaagtg ttatcggtaa
      661 cacagccctc ctggccttga ctttgggacg cacgatcata aacgagaccg ctcgagacca
      721 ccaggataca tagattacca caactccgag cccttccacc cacagaatca ggggataacg
      781 caggaaagaa catgtgagca aaaggccagc aaaaggcaag gaaccgtaaa aaggccgcgt
      841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1261 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1441 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1501 ggattttggt catgcattct aggtgattat tatttgccga ctaccttggt gatctcgcct
     1561 ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct
     1621 tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc
     1681 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
     1741 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
     1801 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
     1861 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
     1921 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga taccagcaag aatgtcattg
     1981 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
     2041 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cactctctcc aggggaagcc
     2101 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
     2161 accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg
     2221 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
     2281 gatagttgag tcgatacttc ggcgatcacc gcttccctca tgcgaaacga tcctcatcct
     2341 gtctcttgat cagatattga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     2401 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     2461 tcgcttgcta agcttgcatg cctgcaagtc gactctagag gagcggtgat cacaggcagc
     2521 aacgctctgt catcgttaca atcaacatgc taccctccgc ga
//
