LOCUS       sequence-405681-        2635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 54101 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-NRT
FEATURES             Location/Qualifiers
     source          1..2635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1293..1881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1373..1392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1972..2635,1..128))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
       61 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
      121 tccctcatgc gaaacgatcc tcatcctgtc tcttgatcag atattgatcc cctgcgccat
      181 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
      241 gagggcgccc cagctggcaa ttccggttcg cttgctaagc ttgcatgcct gcaagtcgac
      301 tctagaggag cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
      361 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
      421 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
      481 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctcattact cgcatccatt
      541 ctcaggctgt ctcgtctcgt ctcaggtctc cgcttagtct cctggttgat tttgtccatt
      601 tcccccgact atttccttca acatcttatt cttaaagtta cttattttct tttctacact
      661 atagcgtatc tatgaacttg tgtaagagag ttgcaacggt tgactgtagt gctcaattga
      721 cacagcgacc ggaaatgccc ttgttaactc taaaaggtgc ttatcctatt taggtccctg
      781 gatttaagga gaacttggat tgatcccccc tccccccacg ggtgcggcac aggtcagcag
      841 atccttgacg attttgaagg acgacagcat acttgcgcaa atttgcgaga tagccttatt
      901 gctgacctac aggtctcctt aaatgcggta ataccgcaaa taaccgcaaa cagcttgttt
      961 gcggtgaatt tcaaaacttg tttgcgccta tttgcggtat tcattgccct gcagctatat
     1021 acttaacatc tttttgtcac atctatgtcc agaaatatat aatatttaca tgaaagcttt
     1081 cttcactttg tgttgacggt tgtatcttag ttttaaagta gtttactcca cgatgagagt
     1141 ggagagagtc cgctcgagac caccaggata catagattac cacaactccg agcccttcca
     1201 cccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1261 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1321 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1381 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1441 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1501 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1561 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1621 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1681 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1741 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1801 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1861 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1921 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt attatttgcc
     1981 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     2041 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     2101 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     2161 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     2221 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     2281 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     2341 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     2401 gataccagca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     2461 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     2521 ctcactctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     2581 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggc
//
