LOCUS       sequence-405684-        2542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 5902 from
            Phaeodactylum tricornutum with EF overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-HO
FEATURES             Location/Qualifiers
     source          1..2542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     382..401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1553..2344
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2435..2542,1..481)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
       61 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      121 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      181 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      241 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      301 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      361 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      421 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      481 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      541 gttctttcct gcgttatccc ctgattctgt gggtggaagg gctcggagtt gtggtaatct
      601 atgtatcctg gtggtctcga gcgggtgact aacaatcctt agagctccgt ctccattttt
      661 tagatgacga tcgcaatatg gcacaggtat cggttctcct tcccgactta ggactaaagt
      721 actacagcgc ctgccggatc tcgtatgagc ccaacagata acaggcttcg aaatcgtcgt
      781 tttgttttga ggcttgacag cgagcgttac cgaatgattg ctagccattc gcatatactg
      841 tcagataatc gtcggatgtt cccctggaaa aattcgcgag ctgacttttt tcagataaat
      901 gctgtcagcc acgaaattgt ttacatatta gtttgtttac caacaagggg ctaacattta
      961 tgtcaaacta ccaagaacag tctaaagctc gctgtcaact tgcagcatga tcaatgatga
     1021 caagacatgt gaatactgac aggagagttc ttttttacga tgcaactcca agttttagct
     1081 tcaaggactt ccaagccaaa gaccaaagcg gagacctgag acgagacgag acagcctgag
     1141 aatggatgcg agtaatgagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     1201 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     1261 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     1321 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaccg ctcctctaga
     1381 gtcgacttgc aggcatgcaa gcttagcaag cgaaccggaa ttgccagctg gggcgccctc
     1441 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
     1501 atggcgcagg ggatcaatat ctgatcaaga gacaggatga ggatcgtttc gcatgaggga
     1561 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     1621 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     1681 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     1741 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gtgagattct
     1801 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     1861 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgctg gtatcttcga
     1921 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     1981 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     2041 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     2101 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     2161 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     2221 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     2281 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     2341 ataataatca cctagaatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2401 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2461 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2521 agctaccaac tctttttccg aa
//
