LOCUS       sequence-405685-        2130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 29812 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-act1_tr
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1093..1884)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
       61 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
      121 tgtatcgagt ggtgattttg tgccgagctc attactcgca tccattctca ggctgtctcg
      181 tctcgtctca ggtctccgct tgctactgag cttcatagtt acagttggtt ccgcagcagg
      241 gaggagcaac aatcttgact ttgatggagg ccgctcgaga ccaccaggat acatagatta
      301 ccacaactcc gagcccttcc acccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
     1081 tctaggtgat tattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
     1141 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
     1201 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     1261 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     1321 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     1381 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     1441 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     1501 gtcgatcgtg gctggctcga agataccagc aagaatgtca ttgcgctgcc attctccaaa
     1561 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     1621 gacttctaca gcgcggagaa tctcactctc tccaggggaa gccgaagttt ccaaaaggtc
     1681 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     1741 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     1801 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     1861 ttcggcgatc accgcttccc tcatgcgaaa cgatcctcat cctgtctctt gatcagatat
     1921 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     1981 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctaagcttgc
     2041 atgcctgcaa gtcgactcta gaggagcggt gatcacaggc agcaacgctc tgtcatcgtt
     2101 acaatcaaca tgctaccctc cgcgagatca
//
