LOCUS       sequence-405687-        2590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 29812 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-act1_full
FEATURES             Location/Qualifiers
     source          1..2590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(725..1313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(805..824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1404..2195)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctcagc ttgctactga
       61 gcttcatagt tacagttggt tcttcaacgt ttgtaggagg tgtattaatc ttttataacg
      121 ctgcctttat atgttcgctt tcttccaagt agagaattaa cggaaatcgt cgtcggttga
      181 gaataacgca gaaagtcctt cgttgtcgag ccgtaaagca gctggctgct ctcattctaa
      241 aagttcacgc tctgcacgtg cgataacgag gggataccgt atacaatcgt ctttgtactg
      301 gaaaagatct tctcaaaatg attggaagaa atcgatttca catataaagg agcgttataa
      361 aagattaata caccgcctac aaacgttgaa gaaccaactg taactatgaa gctcagtagc
      421 ttagaagcac ttgcgatgaa caatagaagg cccagactca tcgtactctt ccttcgagat
      481 ccacatgctc tggaaagtag aaagggaagc gagaattgac cctccaatcc aaacagagta
      541 cttgcgttcc ggaggagcaa caatcttgac tttgatggag gccgctcgag accaccagga
      601 tacatagatt accacaactc cgagcccttc cacccacaga atcaggggat aacgcaggaa
      661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      721 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1141 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1381 tggtcatgca ttctaggtga ttattatttg ccgactacct tggtgatctc gcctttcacg
     1441 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
     1501 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
     1561 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
     1621 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
     1681 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
     1741 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
     1801 gccagatcaa tgtcgatcgt ggctggctcg aagataccag caagaatgtc attgcgctgc
     1861 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
     1921 acaacaatgg tgacttctac agcgcggaga atctcactct ctccagggga agccgaagtt
     1981 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
     2041 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
     2101 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
     2161 tgagtcgata cttcggcgat caccgcttcc ctcatgcgaa acgatcctca tcctgtctct
     2221 tgatcagata ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     2281 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt
     2341 gctaagcttg catgcctgca agtcgactct agaggagcgg tgatcacagg cagcaacgct
     2401 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
     2461 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
     2521 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
     2581 gtgccgagct
//
