LOCUS       sequence-405688-        2547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 21592 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-lysA
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      765..1353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1254..1273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2430..2547,1..674)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
       61 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
      121 gtgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
      181 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgctg
      241 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
      301 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
      361 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
      421 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
      481 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
      541 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
      601 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
      661 tagtcggcaa ataataatca cctagaatgc atgaccaaaa tcccttaacg tgagttttcg
      721 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      781 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      841 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      901 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      961 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1021 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1081 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1141 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1201 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1261 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1321 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1381 ttccttgcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1441 gtgggtggaa gggctcggag ttgtggtaat ctatgtatcc tggtggtctc gagcgtgacg
     1501 gcatcggatg agactctatg aagtcgcaac cataaatcct tttccaccac caacctcaac
     1561 gagaaaacct agccattcaa acatcatacg cgagaaaatg agaaagtgtc gaagagatca
     1621 ttgtgttttc attggctagt ttctttgcta acgtatgccc gaaaaaggtc acaatttgaa
     1681 agtgactgta ttttttcgta gcaaggcgcc ggcctgttat attccgggaa taatagaata
     1741 agcgccttat ccgctcaact ctagctaatc aatcccatca cgcagtcgtt tttctaaacg
     1801 cgacgggaat tcatcgggga tgaagccgca accttgtcca actccgtgga cagcggcaca
     1861 gagtaccgga aacatgcgtc aactggctta actcaatatc agccgcaagc aatacataat
     1921 caataaatct tctaattact gtttgatact gtttctagtc caaacacatt atactgttcc
     1981 ttaagcggag acctgagacg agacgagaca gcctgagaat ggatgcgagt aatgagctcg
     2041 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     2101 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     2161 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     2221 acagagcgtt gctgcctgtg atcaccgctc ctctagagtc gacttgcagg catgcaagct
     2281 tagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
     2341 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaatatctg
     2401 atcaagagac aggatgagga tcgtttcgca tgagggaagc ggtgatcgcc gaagtatcga
     2461 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2521 tacatttgta cggctccgca gtggatg
//
