LOCUS       sequence-405690-        2559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 47434 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-vapol
FEATURES             Location/Qualifiers
     source          1..2559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             410..1201
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1292..1880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1781..1800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggatgcgagt aatgagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
       61 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
      121 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
      181 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgctc ctctagagtc
      241 gacttgcagg catgcaagct tagcaagcga accggaattg ccagctgggg cgccctctgg
      301 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
      361 gcgcagggga tcaatatctg atcaagagac aggatgagga tcgtttcgca tgagggaagc
      421 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      481 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      541 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      601 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagtg agattctccg
      661 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      721 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgctggta tcttcgagcc
      781 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      841 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      901 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      961 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     1021 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     1081 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1141 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1201 ataatcacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1921 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg ggtggaaggg
     1981 ctcggagttg tggtaatcta tgtatcctgg tggtctcgag cgaggtagaa tttctctcca
     2041 tagaagccga gactgcggca atattacgta gacaaacaag ggattgtcgc ccctttcgga
     2101 gtcagagctt tttctttcta accaggctcc agccttggaa ggtacaacga ttgtcacatc
     2161 cattttttgg ttccattgac tctggctagt agtacacttc cgctgcaaag aacatgctta
     2221 ttttctgtac cgcacatttt gcagcgtgga tggtttttga tccggaatgt tggtgggcaa
     2281 tttctcgtcc gtatgtccca cggacaataa cagcgttttg acctcggtct atattccctt
     2341 tcaaaaaggc tcggtacaga ctatatttat aacttggagt acttctacga aataatttta
     2401 caaaacctat ataaagacaa catgcttcca tatgtctttg tttcaagggc ggaacgggga
     2461 actggcaggc attgcacact gcatagccac tggcgacaaa aagatcttcc agctccgggg
     2521 gaagcggaga cctgagacga gacgagacag cctgagaat
//
