LOCUS       sequence-405691-        2506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 23924 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-GPI1
FEATURES             Location/Qualifiers
     source          1..2506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(351..939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(431..450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1030..1821)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tccttgaacc gaagctcctg tctttcgaga tgcgcttagt tgtgagcgta tgtgcgtagc
       61 tagaactttg cccaactcga caccgaactg atcccagcta ttgatacccc atatgaagcc
      121 ttggaccgca gtccgatgtt catacatggc aatcagctgc ccaacggcaa aggcatctaa
      181 tcgtgtcatc agaatactgc tgcttggtcg cttgagacca ccaggataca tagattacca
      241 caactccgag cccttccacc cacagaatca ggggataacg caggaaagaa catgtgagca
      301 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      361 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      421 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      481 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      541 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      601 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      661 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      721 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      781 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      841 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      901 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      961 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     1021 aggtgattat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     1081 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     1141 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     1201 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     1261 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     1321 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     1381 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     1441 gatcgtggct ggctcgaaga taccagcaag aatgtcattg cgctgccatt ctccaaattg
     1501 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     1561 ttctacagcg cggagaatct cactctctcc aggggaagcc gaagtttcca aaaggtcgtt
     1621 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     1681 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     1741 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     1801 ggcgatcacc gcttccctca tgcgaaacga tcctcatcct gtctcttgat cagatattga
     1861 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     1921 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgcta agcttgcatg
     1981 cctgcaagtc gactctagag gagcggtgat cacaggcagc aacgctctgt catcgttaca
     2041 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     2101 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     2161 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctcatt
     2221 actcgcatcc attctcaggc tgtctcgtct cgtctcaggt ctcagcttac tgtagagcat
     2281 tcgagctggg acgtctggcg tgttaagatg caacaaaata aacgatgtga agtaggaagc
     2341 gattccaatg acgcatgaaa gttgtagctc tgcgttatat ccatttaatt tagcaaatcc
     2401 aatcgtaaat atgacgcgtt tacaaagtct tttctgtaga ggtcggctcg gggtgctttc
     2461 catgcgctag aaaatgctct agaagtgaac tcgttgacga gttgaa
//
