LOCUS       sequence-405693-        2496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 34971 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-H4
FEATURES             Location/Qualifiers
     source          1..2496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1644..2232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1724..1743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2323..2496,1..618))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
       61 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
      121 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      181 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      241 cgtggctggc tcgaagatac cagcaagaat gtcattgcgc tgccattctc caaattgcag
      301 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
      361 tacagcgcgg agaatctcac tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
      421 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
      481 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
      541 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
      601 gatcaccgct tccctcatgc gaaacgatcc tcatcctgtc tcttgatcag atattgatcc
      661 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
      721 aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctaagc ttgcatgcct
      781 gcaagtcgac tctagaggag cggtgatcac aggcagcaac gctctgtcat cgttacaatc
      841 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
      901 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
      961 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctcattact
     1021 cgcatccatt ctcaggctgt ctcgtctcgt ctcaggtctc agcttgcttg cgcttgatcc
     1081 tcgactttgt tgctcgtctt taaaaccttg gaacgattat atacaatcac caactcaaaa
     1141 accggatttt ctaaaatcca cccaaacaac caaagaaaat acttctcatt gcatttatga
     1201 atcacagcag acctgcgtcc ttttaaaact tagatcctgt tttcttataa aaaacagatc
     1261 aaattttctg ggagttcatt gactctgcca gtcagaatca atcctgcagt aattctttat
     1321 ttacaggtga aagtaaaaga gaatcccaat tttttgcttg tacattaagg tcctccttac
     1381 attacagcta atttcaaaat aagatgaagt tggattcgtg tcctttcatg gtgatgtgat
     1441 attctgcact ataccaaaca ccgtgaaatg tcagctagag cttgtcatga ggcagttgct
     1501 gcgcttgaga ccaccaggat acatagatta ccacaactcc gagcccttcc acccacagaa
     1561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2281 aactcacgtt aagggatttt ggtcatgcat tctaggtgat tattatttgc cgactacctt
     2341 ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa
     2401 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
     2461 ggccggcagg cgctccattg cccagtcggc agcgac
//
