LOCUS       sequence-405694-        2704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 54983 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-NR
FEATURES             Location/Qualifiers
     source          1..2704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1858..2446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1938..1957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2537..2704,1..624))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
       61 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
      121 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
      181 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
      241 gtcgatcgtg gctggctcga agataccagc aagaatgtca ttgcgctgcc attctccaaa
      301 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
      361 gacttctaca gcgcggagaa tctcactctc tccaggggaa gccgaagttt ccaaaaggtc
      421 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
      481 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
      541 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
      601 ttcggcgatc accgcttccc tcatgcgaaa cgatcctcat cctgtctctt gatcagatat
      661 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
      721 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctaagcttgc
      781 atgcctgcaa gtcgactcta gaggagcggt gatcacaggc agcaacgctc tgtcatcgtt
      841 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
      901 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
      961 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctc
     1021 attactcgca tccattctca ggctgtctcg tctcgtctca ggtctcagct taagttcttg
     1081 actgattgtc atatcaattt tgcactttac aatacatttt caatagcaat ttactttaag
     1141 actagcgcaa tttttttctt acattagatg aacataaaac gacgatgagg atcactgtta
     1201 gggaaacgat gaactacgct gaggacttcc tattattcgt cctttgatgg cgtggttgtt
     1261 ctttggtttg cggtcgagcg caacgaccaa ggcctgtacc aaaaacgaag ttgataatca
     1321 gatatttgag ttgtaacaag aaactaaagc attattttag cagtagcacg aatcaggatc
     1381 taaaatgaac gcaattgcat ggtttatgtc taagctgcgt tagaacttag aaattgaaga
     1441 gaacacgccc ttcgaagacg cccgccttcg tcaggaagtc tttcacagct tcagtcatgc
     1501 ctttcatacc acaaagcaaa gctcctgaat tccgaggaat atcgactccg tcttcctcta
     1561 aagcagtctg aatgtatcca gtccgtccct tccagacgct ttcgtcaggt tgactcaaca
     1621 cgggaacgac ttcgaagccg gcggcttccc actcactgta cttgtccacg taacacaaat
     1681 cttctgccgt gcgctcgccg tagtatagac gagctcgctt gagaccacca ggatacatag
     1741 attaccacaa ctccgagccc ttccacccac agaatcaggg gataacgcag gaaagaacat
     1801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2521 gcattctagg tgattattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
     2581 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
     2641 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
     2701 cggc
//
