LOCUS       sequence-405696-        1872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 3a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK101
FEATURES             Location/Qualifiers
     source          1..1872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(237..271)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(342..1001)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     916..935
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1183..1771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1672..1691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggcctcca ctcgtgtcct cgcctctcgc ctggcctccc agatggctgc
       61 ttccgccaag gttgcccgcc ctgctgtccg cgttgctcag gtcagcaagc gcaccatcca
      121 gactggctcc cccctccaga ccctcaagcg cacccagatg acctccatcg tcaacgccac
      181 cacccgccag gctttccaga agcgcgccta ctcttccggt tcttgagacc agaccaataa
      241 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      301 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      361 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      421 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      481 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      541 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
      601 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
      661 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
      721 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
      781 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
      841 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
      901 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
      961 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1021 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1081 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     1141 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1201 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1261 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1321 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1381 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1441 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1501 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1561 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1621 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1681 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1741 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1801 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1861 ggataaccgt ag
//
