LOCUS       sequence-405698-        2511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 54330 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-dph1
FEATURES             Location/Qualifiers
     source          1..2511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1669..2257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1749..1768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2348..2511,1..628))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
       61 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
      121 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
      181 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
      241 caatgtcgat cgtggctggc tcgaagatac cagcaagaat gtcattgcgc tgccattctc
      301 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
      361 tggtgacttc tacagcgcgg agaatctcac tctctccagg ggaagccgaa gtttccaaaa
      421 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
      481 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
      541 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
      601 atacttcggc gatcaccgct tccctcatgc gaaacgatcc tcatcctgtc tcttgatcag
      661 atattgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
      721 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctaagc
      781 ttgcatgcct gcaagtcgac tctagaggag cggtgatcac aggcagcaac gctctgtcat
      841 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
      901 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
      961 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     1021 gctcattact cgcatccatt ctcaggctgt ctcgtctcgt ctcaggtctc cgcttcatgg
     1081 tcgttcattc atagaagaat cgacttctct tccagatttc gaatgcttgt agctttcgtt
     1141 tattcttcgg ccgcgcagct gctgctatca attcacaatg ttaaaatata taccttcgct
     1201 tccactacat ggtaattaca agcgtggctg gagatagctc gtgtgtattt ctcaaacaca
     1261 cggccagaaa gtctttgcat ctcggcacta gacttttcta tggaagagac atccgaaaga
     1321 atcgccaaat acgtgataaa ataaaattga atgcccacga caaacatggt atttatctct
     1381 aactgtaagc catgacacaa ttttgtcagt ttgggtgcat tttgagatga ggtggaaaga
     1441 gcgtctactt gacagaactt tcgaatagac tacagaatag cgacggagct tctcaagtag
     1501 cgtagcctat ccaagcttct tggcgacgct cgagaccacc aggatacata gattaccaca
     1561 actccgagcc cttccaccca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2101 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
     2341 gtgattatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     2401 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     2461 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag t
//
